Molecular markers of endometrial carcinoma detected in uterine aspirates
Open Access
- 4 January 2011
- journal article
- research article
- Published by Wiley in International Journal of Cancer
- Vol. 129 (10), 2435-2444
- https://doi.org/10.1002/ijc.25901
Abstract
Endometrial cancer (EC) is the most frequent of the invasive tumors of the female genital tract. Although usually detected in its initial stages, a 20% of the patients present with advanced disease. To date, no characterized molecular marker has been validated for the diagnosis of EC. In addition, new methods for prognosis and classification of EC are needed to combat this deadly disease. We thus aimed to identify new molecular markers of EC and to evaluate their validity on endometrial aspirates. Gene expression screening on 52 carcinoma samples and series of real-time quantitative PCR validation on 19 paired carcinomas and normal tissue samples and on 50 carcinoma and noncarcinoma uterine aspirates were performed to identify and validate potential biomarkers of EC. Candidate markers were further confirmed at the protein level by immunohistochemistry and Western blot. We identified ACAA1, AP1M2, CGN, DDR1, EPS8L2, FASTKD1, GMIP, IKBKE, P2RX4, P4HB, PHKG2, PPFIBP2, PPP1R16A, RASSF7, RNF183, SIRT6, TJP3, EFEMP2, SOCS2 and DCN as differentially expressed in ECs. Furthermore, the differential expression of these biomarkers in primary endometrial tumors is correlated to their expression level in corresponding uterine fluid samples. Finally, these biomarkers significantly identified EC with area under the receiver-operating-characteristic values ranging from 0.74 to 0.95 in uterine aspirates. Interestingly, analogous values were found among initial stages. We present the discovery of molecular biomarkers of EC and describe their utility in uterine aspirates. These findings represent the basis for the development of a highly sensitive and specific minimally invasive method for screening ECs.This publication has 15 references indexed in Scilit:
- Subtractive Proteomic Approach to the Endometrial Carcinoma Invasion FrontJournal of Proteome Research, 2009
- Endometrial carcinoma: ESMO Clinical Recommendations for diagnosis, treatment and follow-upAnnals of Oncology, 2009
- Should Preoperative Pathology Be Used to Select Patients for Surgical Staging in Endometrial Cancer?International Journal of Gynecologic Cancer, 2009
- Cancer screening in the United States, 2009: A review of current American Cancer Society guidelines and issues in cancer screeningCA: A Cancer Journal for Clinicians, 2009
- Magnetic Resonance Imaging in Gynecological OncologyInternational Journal of Gynecologic Cancer, 2009
- Novel molecular profiles of endometrial cancer—new light through old windowsThe Journal of Steroid Biochemistry and Molecular Biology, 2008
- Cancer Statistics, 2008CA: A Cancer Journal for Clinicians, 2008
- Development of multimarker panel for early detection of endometrial cancer. High diagnostic power of prolactinGynecologic Oncology, 2007
- A Differential Gene Expression Profile Reveals Overexpression of RUNX1/AML1 in Invasive Endometrioid CarcinomaCancer Research, 2004