Curcumin ameliorates acute thioacetamide‐induced hepatotoxicity
- 24 February 2006
- journal article
- research article
- Published by Wiley in Journal of Gastroenterology and Hepatology
- Vol. 21 (2), 358-366
- https://doi.org/10.1111/j.1440-1746.2005.03984.x
Abstract
Increased production of reactive oxygen species and nitric oxide and activation of nuclear factor kappa B are implicated in the pathogenesis of various liver diseases, including fulminant hepatic failure. Curcumin is a naturally occurring anti-oxidant that reduces oxidative stress and inhibits nuclear factor kappa B and nitric oxide formation. The aim of the present study is to assess curcumin's therapeutic potential in acute thioacetamide hepatotoxicity, a rat model of fulminant hepatic failure. Fulminant hepatic failure was induced by two intraperitoneal (i.p.) injections of 300 mg/kg thioacetamide (TAA) at 24-h intervals. The experimental groups received a low-dose (200 mg/kg per day, i.p.) or a high-dose (400 mg/kg per day) of curcumin, initiated 48 h prior to the first TAA injection. A fourth group was administered neither TAA nor curcumin and served as a control. The survival rate was higher in both curcumin-treated groups compared to the TAA only treated group. Biochemical parameters of liver injury, blood ammonia and hepatic necroinflammation were lower in the low-dose curcumin group compared to TAA controls, and were further reduced in the high-dose group (P < 0.05 and P < 0.01, respectively). Curcumin treatment also reduced the TAA-induced elevated hepatic levels of thiobarbituric acid-reactive substances (TBARS), and inhibited the nuclear binding of nuclear factor kappa B (NFkappaB) and inducible nitric oxide (iNOS) protein expression. Curcumin improved survival and minimized oxidative stress, hepatocellular injury and hepatic necroinflammation, NFkappaB binding and iNOS expression in a rat model of FHF. These findings support the role of ROS, NFkappaB and iNOS in mediating liver insult due to TAA, and that of curcumin as a hepato-protectant.Keywords
This publication has 58 references indexed in Scilit:
- Screening pharmaceutical preparations containing extracts of turmeric rhizome, artichoke leaf, devil's claw root and garlic or salmon oil for antioxidant capacityJournal of Pharmacy and Pharmacology, 2003
- Curcumin, the major component of food flavour turmeric, reduces mucosal injury in trinitrobenzene sulphonic acid‐induced colitisBritish Journal of Pharmacology, 2003
- The Protective Effects of Tetrahydrocurcumin on Oxidative Stress in Cholesterol-fed RabbitsJournal of Atherosclerosis and Thrombosis, 2002
- The curcuma antioxidants: pharmacological effects and prospects for future clinical use. A reviewArchives of Gerontology and Geriatrics, 2001
- Suppressive Effect of Curcumin on Trichloroethylene-induced Oxidative Stress.Journal of Nutritional Science and Vitaminology, 2000
- Effect of Thioacetamide on the Hepatic Expression of γ-Glutamylcysteine Synthetase Subunits in the RatToxicology and Applied Pharmacology, 1999
- NFkappaB prevents apoptosis and liver dysfunction during liver regeneration.JCI Insight, 1998
- Curcumin, an Anti-tumor Promoter and Anti-inflammatory Agent, Inhibits Induction of Nitric Oxide Synthase in Activated MacrophagesBiochemical and Biophysical Research Communications, 1995
- Inhibitory effect of curcumin on xanthine dehydrogenase/oxidase induced by phorbol-12-myristate-13-acetate in NJH3T3 cellsCarcinogenesis: Integrative Cancer Research, 1994
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976