Molecular Characterization of Mutations in Anticarsia gemmatalis Cadherin Gene and their Relation to Bacillus thurigiensis Resistance
Open Access
- 28 April 2022
- journal article
- Published by Avanti Publishers in Global Journal of Agricultural Innovation, Research & Development
- Vol. 9, 54-60
- https://doi.org/10.15377/2409-9813.2022.09.5
Abstract
Anticarsia gemmatalis Hübner, 1818 is the main soybean defoliating pest in Brazil. The biological control of the species is done with products based on toxins produced by Bacillus thurigiensis (Bt), as bioinsecticides, or in transgenic plants. After activation by intestinal proteases, these toxins interact with receptors, especially cadherin, leading to death due to the formation of cellular pores. In recent years resistant populations have been identified in the laboratory, which can be a problem if the same patterns are found in crops, reducing their control effect. In this paper, we performed a comparative structural analysis of a mutation region for the gene of this receptor in A. gemmatalis, among resistant and susceptible strains treated with a toxin produced by Bt (Cry1Ac). The HaCad fragment of the cadherin gene was amplified by PCR, sequenced, and analyzed by bioinformatics tools. The PCR results were positive for resistant specimens but not for susceptible strains, suggesting the presence of a mutation in the resistant strain. In the sequenced fragments of the resistant insects, six haplotypes were found, and the originated amino acid sequences demonstrated the modification in four sites, which did not interfere with the three-dimensional shape of the protein. These data showed considerable variation taking into account the size of the fragment, even if they do not affect the final structure of the protein. The results allowed a better understanding of the mechanisms of resistance to Cry1Ac in the species, mainly in the involvement of cadherin in this process.Keywords
This publication has 21 references indexed in Scilit:
- Bacillus thuringiensis: mechanism of action, resistance, and new applications: a reviewCritical Reviews in Biotechnology, 2014
- Non-Recessive Bt Toxin Resistance Conferred by an Intracellular Cadherin Mutation in Field-Selected Populations of Cotton BollwormPLOS ONE, 2012
- Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and WindowsMolecular Ecology Resources, 2010
- Mitochondrial DNA Diversity of Orchid Bee Euglossa fimbriata (Hymenoptera: Apidae) Populations Assessed by PCR-RFLPBiochemical Genetics, 2010
- Common genomic structure for the Lepidoptera cadherin-like genesGene, 2006
- Estirpes de Bacillus thuringiensis efetivas contra insetos das ordens Lepidoptera, Coleoptera e DipteraPesquisa Agropecuária Brasileira, 2004
- Hydropathic Complementarity Determines Interaction of Epitope 869HITDTNNK876 in Manduca sexta Bt-R1 Receptor with Loop 2 of Domain II ofBacillus thuringiensis Cry1A ToxinsOnline Journal of Public Health Informatics, 2002
- Bt resistance managementNature Biotechnology, 1998
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- Velvetbean Caterpillar: A Rearing Procedure and Artificial Medium123Journal of Economic Entomology, 1976