Tantalum nitride coatings prepared by magnetron sputtering to improve the bioactivity and osteogenic activity for titanium alloy implants
Open Access
- 6 December 2017
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in RSC Advances
- Vol. 7 (87), 55408-55417
- https://doi.org/10.1039/c7ra09032c
Abstract
A good orthopedic load-bearing implant should have both excellent mechanical properties and biological activity. In order to improve the biological activity of Ti6Al4V-based implants, tantalum nitride (TaN) films were deposited on the surface of Ti6Al4V alloy and Si(100) substrates by magnetron sputtering. Characterized by XRD and XPS, it was proved that the uniform cubic NaCl-structure TaN films were successfully prepared on the Ti6Al4V alloy substrate. The TaN film showed higher hardness (19.7 GPa) and elastic modulus (254.4 GPa) compared with HA coatings and Ti6Al4V alloys. We evaluated cytocompatibility and osteogenesis activity of the TaN coatings using rabbit bone marrow stromal cells (BMSCs) cultured on the coated and uncoated samples. The biocompatibility of the films and their effect on cell proliferation were examined by live/dead cell staining and CCK-8 assay. SEM and cytoskeleton fluorescent staining were used to observe the cell adhesion and morphology on different samples. The effect of the film on osteoblastic activity was assessed by calcium nodule staining and alkaline phosphatase quantitative assays. We demonstrate that TaN film can enhance the adhesion, proliferation, and differentiation of BMSCs. These results suggest that TaN film prepared by magnetron sputtering has great potential to be applied in orthopedics.Funding Information
- China Postdoctoral Science Foundation (2016M600229)
- National Natural Science Foundation of China (51602122)
This publication has 44 references indexed in Scilit:
- Porous Tantalum Coatings Prepared by Vacuum Plasma Spraying Enhance BMSCs Osteogenic Differentiation and Bone Regeneration In Vitro and In VivoPLOS ONE, 2013
- Synthesis of Ordered Porous Graphitic‐C3N4 and Regularly Arranged Ta3N5 Nanoparticles by Using Self‐Assembled Silica Nanospheres as a Primary TemplateChemistry – An Asian Journal, 2010
- Effects of titanium surface topography on bone integration: a systematic reviewClinical Oral Implants Research, 2009
- Microstructure and mechanical behavior of Ti–6Al–4V produced by rapid-layer manufacturing, for biomedical applicationsJournal of the Mechanical Behavior of Biomedical Materials, 2009
- Superior Osteogenic Capacity for Bone Tissue Engineering of Fetal Compared with Perinatal and Adult Mesenchymal Stem CellsThe International Journal of Cell Cloning, 2009
- How useful is SBF in predicting in vivo bone bioactivity?Biomaterials, 2006
- Characterization of adhesion and differentiation markers of osteogenic marrow stromal cellsJournal of Cellular Physiology, 2004
- Chemical vapor deposition growth and properties of TaCxNyThin Solid Films, 2002
- Caspases: opening the boxes and interpreting the arrowsCell Death & Differentiation, 2002
- An improved technique for determining hardness and elastic modulus using load and displacement sensing indentation experimentsJournal of Materials Research, 1992