Loop-mediated isothermal amplification (LAMP): a versatile technique for detection of micro-organisms
Top Cited Papers
- 11 February 2018
- journal article
- review article
- Published by Oxford University Press (OUP) in Journal of Applied Microbiology
- Vol. 124 (3), 626-643
- https://doi.org/10.1111/jam.13647
Abstract
Loop mediated isothermal amplification (LAMP) amplifies DNA with high specificity, efficiency, and rapidity under isothermal conditions by using a DNA polymerase with high displacement strand activity and a set of specifically designed primers to amplify targeted DNA strands. Following its first discovery by Notomi et al. in the year 2000, LAMP was further developed over the years which involved the combination of this technique with other molecular approaches, such as reverse transcription and multiplex amplification for the detection of infectious diseases caused by microorganisms in humans, livestock and plants. In this review, available types of LAMP techniques will be discussed together with their applications in detection of various microorganisms’. Up to date, there are varieties of LAMP detection methods available including colorimetric and fluorescent detection, real-time monitoring using turbidity meter and detection using lateral flow device which will also be highlighted in this review. Apart from that, commercialization of LAMP technique had also been reported such as lyophilized form of LAMP reagents kit and LAMP primer sets for detection of pathogenic microorganisms. On top of that, advantages and limitations of this molecular detection method are also described together with its future potential as a diagnostic method for infectious disease.Keywords
Funding Information
- Ministry of Higher Education Malaysia (UPM/700-2/1/LRGS/5526400)
- Universiti Putra Malaysia
This publication has 166 references indexed in Scilit:
- Rapid and sensitive detection ofVibrio harveyiby loop-mediated isothermal amplification combined with lateral flow dipstick targeted tovhhP2geneAquaculture Research, 2013
- Real-Time Fluorescence Loop Mediated Isothermal Amplification for the Detection of Acinetobacter baumanniiPLOS ONE, 2013
- Isothermal Amplification Methods for the Detection of Nucleic Acids in Microfluidic DevicesBiosensors, 2012
- Electric LAMP: Virtual Loop-Mediated Isothermal AMPlificationISRN Bioinformatics, 2012
- Detection of Toxigenic Clostridium difficile: Comparison of the Cell Culture Neutralization, Xpert C. difficile, Xpert C. difficile/Epi, and Illumigene C. difficile AssaysJournal of Clinical Microbiology, 2012
- Development and evaluation of reverse transcription-loop-mediated isothermal amplification assay for rapid detection of enterovirus 71BMC Infectious Diseases, 2011
- LAMP for Human African Trypanosomiasis: A Comparative Study of Detection FormatsPLoS Neglected Tropical Diseases, 2010
- Rapid detection of pathogenic fungi using loop-mediated isothermal amplification, exemplified by Fonsecaea agents of chromoblastomycosisJournal of Microbiological Methods, 2010
- Development of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of BK VirusJournal of Clinical Microbiology, 2007
- Detection of Loop-Mediated Isothermal Amplification Reaction by Turbidity Derived from Magnesium Pyrophosphate FormationBiochemical and Biophysical Research Communications, 2001