Mesoporous Silica Templated Biolabels with Releasable Fluorophores for Immunoassays
- 6 June 2008
- journal article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 80 (14), 5401-5406
- https://doi.org/10.1021/ac800430m
Abstract
A novel class of fluorescent immuno-biolabeling systems with extremely high F/P ratio (approximately 1000-6000) were prepared by combining the template method and layer-by-layer (LbL) technique. Labels were constructed by loading organic dye fluorescein diacetate (FDA) molecules onto hollow periodic mesoporous organosilica (H-PMO) particles followed by ployelectrolyte encapsulation and antibody attachment. The labeling systems were stimuli-responsive to the addition of concentrated NaOH with the loaded dye molecules being released and detected in a well-controlled manner. When applied in sandwich immunoassays, results indicated that the biolabels were immuno-active and generated an optimal signal that was approximately 50 times higher than the conventional dye labelled antibody system.This publication has 21 references indexed in Scilit:
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