Complete amino acid sequence of the large subunit of the low‐Ca2+‐requiring form of human Ca2+‐activated neutral protease (μCANP) deduced from its cDNA sequence

Abstract

The complete amino acid sequence of the large subunit (catalytic subunit) of human low-Ca²⁺ requiring-calcium-activated neutral protease (μCANP) was deduced from its cDNA base sequence. It is composed of 714 amino acid residues and its sequence is highly homologous to the chicken CANP sequence determined previously. Human μCANP, like chicken CANP, has a clear 4-domain structure, and their fundamental structures are essentially the same, although their Ca²⁺sensitivities are significantly different. The role of each domain in the Ca²⁺sensitivity and protease activity of CANP is discussed on the basis of sequence comparison.