Dehydrin gene expression provides an indicator of low temperature and drought stress: transcriptome-based analysis of Barley (Hordeum vulgare L.)
- 30 May 2008
- journal article
- Published by Springer Science and Business Media LLC in Functional & Integrative Genomics
- Vol. 8 (4), 387-405
- https://doi.org/10.1007/s10142-008-0081-z
Abstract
Low temperature and drought have major influences on plant growth and productivity. To identify barley genes involved in responses to these stresses and to specifically test the hypothesis that the dehydrin (Dhn) multigene family can serve as an indicator of the entire transcriptome response, we investigated the response of barley cv. Morex to: (1) gradual drought over 21 days and (2) low temperature including chilling, freeze–thaw cycles, and deacclimation over 33 days. We found 4,153 genes that responded to at least one component of these two stress regimes, about one fourth of all genes called “present” under any condition. About 44% (1,822 of 4,153) responded specifically to drought, whereas only 3.8% (158 of 4,153) were chilling specific and 2.8% (119 of 4,153) freeze–thaw specific, with 34.1% responsive to freeze–thaw and drought. The intersection between chilling and drought (31.9%) was somewhat smaller than the intersection between freeze–thaw and drought, implying an element of osmotic stress response to freeze–thaw. About 82.4% of the responsive genes were similar to Arabidopsis genes. The expression of 13 barley Dhn genes mirrored the global clustering of all transcripts, with specific combinations of Dhn genes providing an excellent indicator of each stress response. Data from these studies provide a robust reference data set for abiotic stress.Keywords
This publication has 68 references indexed in Scilit:
- Global genome expression analysis of rice in response to drought and high-salinity stresses in shoot, flag leaf, and paniclePlant Molecular Biology, 2007
- Cold Nights Impair Leaf Growth and Cell Cycle Progression in Maize through Transcriptional Changes of Cell Cycle GenesPlant Physiology, 2007
- Barley transcript profiles under dehydration shock and drought stress treatments: a comparative analysisJournal of Experimental Botany, 2006
- TRANSCRIPTIONAL REGULATORY NETWORKS IN CELLULAR RESPONSES AND TOLERANCE TO DEHYDRATION AND COLD STRESSESAnnual Review of Plant Biology, 2006
- Transcriptome Analysis of Cold Acclimation in Barley Albina and Xantha MutantsPlant Physiology, 2006
- Abiotic Stress in Rice. An “Omic” ApproachPlant Physiology, 2006
- An atlas of gene expression from seed to seed through barley developmentFunctional & Integrative Genomics, 2006
- Expression analysis of barley (Hordeum vulgare L.) during salinity stressFunctional & Integrative Genomics, 2006
- A Global Survey of Gene Regulation during Cold Acclimation in Arabidopsis thalianaPLoS Genetics, 2005
- The wheat wcs120 gene family. A useful model to understand the molecular genetics of freezing tolerance in cerealsPhysiologia Plantarum, 1997