Metabolic pathways in tropical dicotyledonous albuminous seeds: Coffea arabica as a case study
Open Access
- 22 January 2009
- journal article
- research article
- Published by Wiley in New Phytologist
- Vol. 182 (1), 146-162
- https://doi.org/10.1111/j.1469-8137.2008.02742.x
Abstract
• The genomic era facilitates the understanding of how transcriptional networks are interconnected to program seed development and filling. However, to date, little information is available regarding dicot seeds with a transient perisperm and a persistent, copious endosperm. Coffea arabica is the subject of increasing genomic research and is a model for nonorthodox albuminous dicot seeds of tropical origin. • The aim of this study was to reconstruct the metabolic pathways involved in the biosynthesis of the main coffee seed storage compounds, namely cell wall polysaccharides, triacylglycerols, sucrose, and chlorogenic acids. For this purpose, we integrated transcriptomic and metabolite analyses, combining real-time RT-PCR performed on 137 selected genes (of which 79 were uncharacterized in Coffea) and metabolite profiling. • Our map-drawing approach derived from model plants enabled us to propose a rationale for the peculiar traits of the coffee endosperm, such as its unusual fatty acid composition, remarkable accumulation of chlorogenic acid and cell wall polysaccharides. • Comparison with the developmental features of exalbuminous seeds described in the literature revealed that the two seed types share important regulatory mechanisms for reserve biosynthesis, independent of the origin and ploidy level of the storage tissue.Keywords
This publication has 65 references indexed in Scilit:
- Comparison of the Effectiveness of Fatty Acids, Chlorogenic Acids, and Elements for the Chemometric Discrimination of Coffee (Coffea arabica L.) Varieties and Growing OriginsJournal of Agricultural and Food Chemistry, 2008
- Transcriptional Profiling of the Arabidopsis EmbryoPlant Physiology, 2006
- Biochemical and genomic analysis of sucrose metabolism during coffee (Coffea arabica) fruit developmentJournal of Experimental Botany, 2006
- Antibodies against a Gossypium hirsutum recombinant cellulose synthase (Ces A) specifically label cellulose synthase in Micrasterias denticulataCellulose, 2006
- The reb1-1 Mutation of Arabidopsis. Effect on the Structure and Localization of Galactose-Containing Cell Wall PolysaccharidesPlant Physiology, 2006
- Coffee and tomato share common gene repertoires as revealed by deep sequencing of seed and cherry transcriptsTheoretical and Applied Genetics, 2005
- A gene expression map of Arabidopsis thaliana developmentNature Genetics, 2005
- The Biosynthesis of UDP-Galacturonic Acid in Plants. Functional Cloning and Characterization of Arabidopsis UDP-d-Glucuronic Acid 4-EpimerasePlant Physiology, 2004
- Trigonelline and sucrose diversity in wild Coffea speciesFood Chemistry, 2004
- High‐resolution histographical mapping of glucose concentrations in developing cotyledons ofVicia fabain relation to mitotic activity and storage processes: glucose as a possible developmental triggerThe Plant Journal, 1998