Activation of conventional protein kinase C (PKC) is critical in the generation of human neutrophil extracellular traps
Open Access
- 1 January 2013
- journal article
- research article
- Published by Springer Science and Business Media LLC in Journal of Inflammation
- Vol. 10 (1), 12-8
- https://doi.org/10.1186/1476-9255-10-12
Abstract
Activation of NADPH oxidase is required for neutrophil extracellular trap (NET) formation. Protein kinase C (PKC) is an upstream mediator of NADPH oxidase activation and thus likely to have a role in NET formation. Pharmacological inhibitors were used to block PKC activity in neutrophils harvested from healthy donor blood. Pan PKC inhibition with Ro-31-8220 (p<0.001), conventional PKC inhibition with Go 6976 (p<0.001) and specific PKCβ inhibition with LY333531 (p<0.01) blocked NET formation in response to PMA. Inhibition of novel and atypical PKC had no effect. LY333531 blocked NET induction by the diacylglycerol analogue OAG (conventional PKC activator) (p<0.001). Conventional PKCs have a prominent role in NET formation. Furthermore PKCβ is the major isoform implicated in NET formation.Keywords
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