The Neglected Marine Fungi, Sensu stricto, and Their Isolation for Natural Products’ Discovery
Open Access
- 10 January 2019
- journal article
- review article
- Published by MDPI AG in Marine Drugs
- Vol. 17 (1), 42
- https://doi.org/10.3390/md17010042
Abstract
Despite the rapid development of molecular techniques relevant for natural product research, culture isolates remain the primary source from which natural products chemists discover and obtain new molecules from microbial sources. Techniques for obtaining and identifying microbial isolates (such as filamentous fungi) are thus of crucial importance for a successful natural products’ discovery program. This review is presented as a “best-practices guide” to the collection and isolation of marine fungi for natural products research. Many of these practices are proven techniques used by mycologists for the isolation of a broad diversity of fungi, while others, such as the construction of marine baiting stations and the collection and processing of sea foam using dilution to extinction plating techniques, are methodological adaptations for specialized use in marine/aquatic environments. To this day, marine fungi, Sensu stricto, remain one of the few underexplored resources of natural products. Cultivability is one of the main limitations hindering the discovery of natural products from marine fungi. Through encouraged collaboration with marine mycologists and the sharing of historically proven mycological practices for the isolation of marine fungi, our goal is to provide natural products chemists with the necessary tools to explore this resource in-depth and discover new and potentially novel natural products.Keywords
This publication has 61 references indexed in Scilit:
- Diverse Lifestyles and Strategies of Plant Pathogenesis Encoded in the Genomes of Eighteen Dothideomycetes FungiPLoS Pathogens, 2012
- Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for FungiProceedings of the National Academy of Sciences of the United States of America, 2012
- Systematic Search for Cultivatable Fungi That Best Deconstruct Cell Walls of Miscanthus and Sugarcane in the FieldApplied and Environmental Microbiology, 2011
- Use of Ichip for High-Throughput In Situ Cultivation of “Uncultivable” Microbial SpeciesApplied and Environmental Microbiology, 2010
- Molecular systematics of the marine DothideomycetesStudies in Mycology, 2009
- A trap for in situ cultivation of filamentous actinobacteriaJournal of Microbiological Methods, 2008
- Detection and Identification of Fungi Intimately Associated with the Brown SeaweedFucus serratusApplied and Environmental Microbiology, 2008
- Marine fungal lineages in the HypocreomycetidaeMycological Research, 2007
- Comparison of protein coding gene contents of the fungal phyla Pezizomycotina and SaccharomycotinaBMC Genomics, 2007
- Illustrated Key to the Filamentous Higher Marine FungiBotanica Marina, 1991