Thrombocyte apheresis cassettes as a novel source of viable peripheral blood mononuclear cells
Open Access
- 14 March 2020
- journal article
- research article
- Published by Wiley in Transfusion
- Vol. 60 (7), 1500-1507
- https://doi.org/10.1111/trf.15756
Abstract
BACKGROUND Traditionally, white blood cells (WBCs) are collected from buffy coats or freshly drawn blood. However, the increasing demand for peripheral blood mononuclear cells (PBMCs) in the research phases of immunological therapy development makes it necessary to identify alternative sources of these cells. STUDY DESIGN AND METHODS Leukapheresis products are cost intensive and not offered by all blood banks. Therefore, thrombocyte apheresis cassettes (TACs), plateletpheresis waste products, were investigated as a possible low‐cost and easily accessible blood source for research laboratories. The recovery rate, phenotype, and functionality of WBC subsets from TAC are unknown and were investigated in comparison to frequently used blood resources via flow cytometry. RESULTS On average, TACs provide 30.3 × 106/mL PBMCs, situating themselves between peripheral whole blood (WB; 5.35 × 106/mL) and leukoreduction system chamber (LRSC; 163.9 × 106/mL) yields. Frequencies of CD14, CD3, CD4, CD8, CD56, CD19, and CD11c positive cells in TACs correlate with normal proportions of WBC populations. Stimulation of TAC‐derived PBMCs by lipopolysaccharide (LPS) and resiquimod (R848) showed no significant differences in expression levels of human leukocyte antigen (HLA)‐DR, DQ, DP, and CD86 or cytokine secretion compared to other blood source derived PBMC. Following stimulation with LPS or R848, comparable levels of tumor necrosis factor‐α, interleukin‐10, and interleukin‐1β could be measured between TAC, LRSC, and WB. Additionally, TAC‐derived T cells retained their proliferation capability and were able to produce interferon‐γ following T‐cell receptor stimulation. CONCLUSION TACs provide a cost‐effective source of viable and functional human blood cells that can readily be used for clinical and laboratory investigations after plateletpheresis preparation.This publication has 15 references indexed in Scilit:
- Cryopreservation-related loss of antigen-specific IFNγ producing CD4+ T-cells can skew immunogenicity data in vaccine trials: Lessons from a malaria vaccine trial substudyVaccine, 2017
- Mature dendritic cell derived from cryopreserved immature dendritic cell shows impaired homing ability and reduced anti-viral therapeutic effectsScientific Reports, 2016
- TCR-mediated functions are enhanced in activated peripheral blood T cells isolated from leucocyte reduction systemsJournal of Immunological Methods, 2015
- Loss of T cell responses following long-term cryopreservationJournal of Immunological Methods, 2007
- Recovery of white blood cells and platelets from leukoreduction system chambers of Trima Accel and COBE Spectra plateletpheresis devicesTransfusion, 2007
- Characterization of mononuclear cells remaining in the leukoreduction system chambers of apheresis instruments after routine platelet collection: a new source of viable human blood cellsTransfusion, 2007
- A novel source of viable peripheral blood mononuclear cells from leukoreduction system chambersTransfusion, 2006
- Analysis of leukocyte binding to depletion filters: role of passive binding, interaction with platelets, and plasma componentsAnnals of Hematology, 2005
- Transfusion of leukoreduced red blood cells may decrease postoperative infections: two meta-analyses of randomized controlled trialsCanadian Journal of Anesthesia/Journal canadien d'anesthésie, 2004
- Leukoreduction of blood componentsCurrent Opinion in Hematology, 2002