Apoptotic cell-free DNA promotes inflammation in haemodialysis patients
Open Access
- 13 December 2011
- journal article
- research article
- Published by Oxford University Press (OUP) in Nephrology Dialysis Transplantation
- Vol. 27 (3), 902-905
- https://doi.org/10.1093/ndt/gfr695
Abstract
A proinflammatory environment characterized by the continuous activation of the innate immune system is thought to contribute to the markedly elevated mortality in haemodialysis (HD) patients with end-stage renal disease (ESRD). The presence of circulating cell-free DNA (cfDNA) has been demonstrated as biomarker in many pathologies. We evaluated the occurrence of cfDNA in HD patients and its functional relevance for innate immunity and inflammation. Here, we found that cfDNA was enhanced in the plasma of ESRD patients after HD compared to healthy controls. Functionally, cfDNA selectively stimulated the production of the proinflammatory cytokine interleukin (IL)-6 by human monocytes, whereas tumour necrosis factor-α or IL-10 was not induced. Conversely, plasma from HD patients, but not from healthy controls or DNase I-treated HD plasma, induced IL-6 production from monocytes. We provide the first evidence that cfDNA has selective immunostimulatory effects on human monocytes. This process may contribute to the proinflammatory milieu observed in HD patients.Keywords
This publication has 20 references indexed in Scilit:
- Analysing cell‐free plasma DNA and SLE disease activityEuropean Journal of Clinical Investigation, 2010
- Cell-free DNA in the blood as a solid tumor biomarker—A critical appraisal of the literatureClinica Chimica Acta; International Journal of Clinical Chemistry, 2010
- Inflammatory Pattern in HemodiafiltrationContributions to nephrology, 2008
- Inflammatory Syndrome in Patients on HemodialysisJournal of the American Society of Nephrology, 2006
- Increased Levels of Soluble TNF-α Receptors and Cellular Adhesion Molecules in Patients Undergoing Bioincompatible HemodialysisAmerican Journal of Nephrology, 2006
- Cell-Free Plasma DNA: A Marker for Apoptosis during HemodialysisClinical Chemistry, 2006
- Increase in and clearance of cell-free plasma DNA in hemodialysis quantified by real-time PCRcclm, 2006
- Increased Serum Levels of Interleukin-12 May Be Associated with Th1 Differentiation in Hemodialysis PatientsNephron, 2002
- Interleukin-6 predicts hypoalbuminemia, hypocholesterolemia, and mortality in hemodialysis patientsAmerican Journal of Kidney Diseases, 1998
- The immune system in end-stage renal diseaseCurrent Opinion in Nephrology and Hypertension, 1993