Characterization of the mouse analogues of CD59 using novel monoclonal antibodies: tissue distribution and functional comparison
Open Access
- 23 April 2003
- journal article
- research article
- Published by Wiley in Immunology
- Vol. 109 (1), 117-126
- https://doi.org/10.1046/j.1365-2567.2003.01628.x
Abstract
CD59, the sole membrane regulator of the membrane attack complex of complement, is broadly and abundantly expressed in man and other mammals. In mouse, CD59 is encoded by two homologous genes. The expression patterns and functional roles of the proteins encoded by these genes, mCD59a and mCD59b, have not been well characterized. Here we describe the generation of monoclonal and polyclonal antibodies detecting specifically mCD59a and mCD59b. These reagents have been used to study function and to ascertain the cell and tissue distributions of mCD59a and mCD59b. mCD59a was broadly distributed on endothelia, erythrocytes, platelets, and on numerous other cell types in organs, a distribution pattern resembling that of CD59 in other species. In marked contrast, expression of mCD59b was restricted to germ cell elements in the testis and mature spermatozoa. Both mCD59a and CD59b inhibited human and rodent complement with similar efficiency. These findings demonstrate that the broadly distributed mCD59a is the key regulator of the terminal complement pathway in mice whereas CD59b, expressed only in testis and on sperm, probably plays other roles in vivo.Keywords
This publication has 26 references indexed in Scilit:
- Human and rodent decay‐accelerating factors (CD55) are not species restricted in their complement‐inhibiting activitiesImmunology, 2000
- Tissue distribution of the rat analogue of decay‐accelerating factorImmunology, 1999
- Characterizationin vitroandin vivoof the pig analogue of human CD59 using new monoclonal antibodiesImmunology, 1998
- The Glycosylation of the Complement Regulatory Protein, Human Erythrocyte CD59Published by Elsevier BV ,1997
- Mutational Analysis of the Active Site and Antibody Epitopes of the Complement-inhibitory Glycoprotein, CD59The Journal of Experimental Medicine, 1997
- Primate terminal complement inhibitor homologues of human CD59Immunogenetics, 1995
- Membrane defence against complement lysis: The structure and biological properties of CD59Immunologic Research, 1993
- The preparation and characterization of monoclonal antibodies to human complement component C8 and their use in purification of C8 and C8 subunitsBiochemical Journal, 1988
- Membrane attack by complementMolecular Immunology, 1984
- Initiation of complement activationSpringer Seminars in Immunopathology, 1984