Absorption of Proteins and Peptides in the Far Ultraviolet
- 9 January 1970
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 167 (3915), 179-181
- https://doi.org/10.1126/science.167.3915.179
Abstract
An absorption peak for the peptide bond at 187 nanometers has been confirmed; a protein assay at this wavelength allows quantitation of proteins in aqueous solution at concentrations between 0.1 and 25 micrograms per milliliter. Assays are conducted at 6°C to take advantage of the reduction of end-absorption of water with temperature.Keywords
This publication has 9 references indexed in Scilit:
- Far Ultraviolet Spectroscopy. II. Analytical ApplicationsApplied Spectroscopy, 1961
- Ultra-Violet Absorption Spectra of the Molecules H2O, HDO and D2ONature, 1960
- The spectrophotometric determination of protein at 210mμBiochemical Journal, 1959
- [73] Spectrophotometric and turbidimetric methods for measuring proteinsMethods in enzymology, 1957
- THE ABSORPTION SPECTRA OF AMINO ACIDS IN THE REGION TWO HUNDRED TO TWO HUNDRED AND THIRTY MILLIMICRONSJournal of Biological Chemistry, 1952
- Far U.V. Spectra of PeptidesThe Journal of Chemical Physics, 1952
- THE ULTRAVIOLET ABSORPTION SPECTRA OF PROTEINSJournal of Biological Chemistry, 1951
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951
- False Absorption Bands in the Region of 200-230 mµ Caused by Stray Radiation in the Beckman SpectrophotometerScience, 1951