Targeted repair of heart injury by stem cells fused with platelet nanovesicles
- 31 December 2017
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Biomedical Engineering
- Vol. 2 (1), 17-26
- https://doi.org/10.1038/s41551-017-0182-x
Abstract
Stem cell transplantation, as used clinically, suffers from low retention and engraftment of the transplanted cells. Inspired by the ability of platelets to recruit stem cells to sites of injury on blood vessels, we hypothesized that platelets might enhance the vascular delivery of cardiac stem cells (CSCs) to sites of myocardial infarction injury. Here, we show that CSCs with platelet nanovesicles fused onto their surface membranes express platelet surface markers that are associated with platelet adhesion to injury sites. We also find that the modified CSCs selectively bind collagen-coated surfaces and endothelium-denuded rat aortas, and that in rat and porcine models of acute myocardial infarction the modified CSCs increase retention in the heart and reduce infarct size. Platelet-nanovesicle-fused CSCs thus possess the natural targeting and repairing ability of their parental cell types. This stem cell manipulation approach is fast, straightforward and safe, does not require genetic alteration of the cells, and should be generalizable to multiple cell types.Keywords
This publication has 36 references indexed in Scilit:
- Circulating platelet-progenitor cell coaggregate formation is increased in patients with acute coronary syndromes and augments recruitment of CD34+ cells in the ischaemic microcirculationEuropean Heart Journal, 2013
- Brief Report: Mechanism of Extravasation of Infused Stem CellsThe International Journal of Cell Cloning, 2012
- Magnetic Enhancement of Cell Retention, Engraftment, and Functional Benefit after Intracoronary Delivery of Cardiac-Derived Stem Cells in a Rat Model of Ischemia/ReperfusionCell Transplantation, 2012
- Direct Comparison of Different Stem Cell Types and Subpopulations Reveals Superior Paracrine Potency and Myocardial Repair Efficacy With Cardiosphere-Derived CellsJournal of the American College of Cardiology, 2012
- Stem and progenitor cell-based therapy in ischaemic heart disease: promise, uncertainties, and challengesEuropean Heart Journal, 2011
- Intramyocardial Injection of Autologous Cardiospheres or Cardiosphere-Derived Cells Preserves Function and Minimizes Adverse Ventricular Remodeling in Pigs With Heart Failure Post-Myocardial InfarctionJournal of the American College of Cardiology, 2011
- Cardiospheres Recapitulate a Niche-Like Microenvironment Rich in Stemness and Cell-Matrix Interactions, Rationalizing Their Enhanced Functional Potency for Myocardial RepairThe International Journal of Cell Cloning, 2010
- Magnetic Targeting Enhances Engraftment and Functional Benefit of Iron-Labeled Cardiosphere-Derived Cells in Myocardial InfarctionCirculation Research, 2010
- Regenerative Potential of Cardiosphere-Derived Cells Expanded From Percutaneous Endomyocardial Biopsy SpecimensCirculation, 2007
- Activated platelets contribute importantly to myocardial reperfusion injuryAmerican Journal of Physiology-Heart and Circulatory Physiology, 2006