Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells
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Open Access
- 12 March 2020
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 117 (13), 7001-7003
- https://doi.org/10.1073/pnas.2002589117
Abstract
A novel betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused a large respiratory outbreak in Wuhan, China in December 2019, is currently spreading across many countries globally. Here, we show that a TMPRSS2-expressing VeroE6 cell line is highly susceptible to SARS-CoV-2 infection, making it useful for isolating and propagating SARS-CoV-2. Our results reveal that, in common with SARS- and Middle East respiratory syndrome-CoV, SARS-CoV-2 infection is enhanced by TMPRSS2.Keywords
Funding Information
- Japan Agency for Medical Research and Development (JP19fk0108104)
- Japan Agency for Medical Research and Development (JP19fk0108030j0403)
- Japan Agency for Medical Research and Development (JP19fk0108058j0802)
- MEXT | Japan Society for the Promotion of Science (17K08868)
- MEXT | Japan Society for the Promotion of Science (18H02665)
- Japan Agency for Medical Research and Development (19fk0108111j0001)
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