Synthesis and Incorporation of 2′-O-Methyl-Pseudouridine into Oligonucleotides
- 1 July 1997
- journal article
- Published by Informa UK Limited in Nucleosides and Nucleotides
- Vol. 16 (7), 1547-1549
- https://doi.org/10.1080/07328319708006226
Abstract
A short multigram synthesis of 2′-O-methylpseudouridine and its phosphoramidite derivative is described which avoids the use of protecting groups on the nitrogens. A binding study of oligonucleotides containing this modification suggest an increased binding affinity to RNA when compared to oligonucleotides incorporating 2′-O-methyluridine.Keywords
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