Rabbit alpha‐fetoprotein: normal levels and breakage tolerance with haptenated homologous alpha‐fetoprotein
- 1 January 1975
- journal article
- research article
- Published by Wiley in European Journal of Immunology
- Vol. 5 (1), 7-10
- https://doi.org/10.1002/eji.1830050103
Abstract
Nanogram quantities of alpha-fetoprotein (AFP), a tumor associated fetal protein, were found in the serum of normal adult rabbits by radioimmunoassay. This AFP was isolated and shown to be immunologically identical to fetal AFP by immunodiffusion. Immunizations of rabbits with unmodified or desialylated AFP in complete Freund's adjuvant did not cause antibody formation, indicating the existance of tolerance against homologous AFP. The tolerance could be terminated by immunizing with hapten-coupled AFP, which resulted in production of rabbit antibodies reacting with unmodified rabbit AFP.Keywords
This publication has 19 references indexed in Scilit:
- Novel human serum protein from fibroblast plasma membraneNature, 1974
- High level of alpha-fetoprotein in sera of adult miceInternational Journal of Cancer, 1973
- Rat α-fetoprotein—III. Refinement of radioimmunoassay for detection of 1 ng rat α1FImmunochemistry, 1973
- Rabbit α-fetoprotein: Immunochemical purification and partial characterizationImmunochemistry, 1973
- Radioimmunoassay for Measurement of Thyroglobulin in Human SerumJCI Insight, 1973
- The specificity of cellular immunity: studies in guinea pigs using defined tetrapeptides containing p‐azobenzenearsonate‐L‐tyrosineEuropean Journal of Immunology, 1973
- The immune response against hapten‐autologous protein conjugates in the mouse. I. Specificity of antibodies produced during the primary response against dinitrophenylated mouse serum albuminEuropean Journal of Immunology, 1973
- Immunological cross-reactions within a family of related haptensImmunochemistry, 1972
- NORMAL AND INCREASED ALPHA-FETOPROTEIN IN NEOPLASTIC AND NON-NEOPLASTIC LIVER DISEASEThe Lancet, 1972
- AN ALTERATION IN TUMOR HISTOCOMPATIBILITY INDUCED BY NEURAMINIDASETransplantation, 1967