Deuterated Solvents as Mobile Phase in Micro-HPLC

Abstract

The chromatographic performance of deuterated solvents, heavy water and deuteriobenzene, has been investigated in reversed phase and normal phase micro-HPLC. The performance of heavy water in separation was comparable or superior to that of light water in reversed phase mode. A slight improvement was also observed in catecholamines separation with heavy water. The performance of deuteriobenzene was observed as complicated in metal complexes separation, but a little difference was present. From those results, the combinations of micro HPLC and IR via flow-cell technique, proton-NMR, and ICP could be accomplished free from absorption or signals from mobile phase solvents.