Geographical variation in antibiotic resistance profiles of Escherichia coli isolated from swine, poultry, beef and dairy cattle farm water retention ponds in Florida1
Open Access
- 29 November 2005
- journal article
- Published by Wiley in Journal of Applied Microbiology
- Vol. 100 (1), 50-57
- https://doi.org/10.1111/j.1365-2672.2005.02773.x
Abstract
Aims: The aim of this study was to assess geographical variation in multiple antibiotic resistance (MAR) profiles of livestock Escherichia coli as well as to evaluate the ability of MAR profiles to differentiate sources of faecal pollution. Methods and Results: More than 2000 E. coli isolates were collected from water retention ponds and manure of swine, poultry, beef and dairy farms in south, central and north Florida, and analysed for MAR using nine antibiotics. There were significant differences in antibiotic resistance of E. coli by season and livestock type for more than one antibiotic, but regional differences were significant only for ampicillin. Over the three regions, discriminant analysis using MAR profiles correctly classified 27% of swine, 49% of poultry, 56% of beef and 51% of dairy isolates. Conclusions: Regional variations in MAR combined with moderate discrimination success suggest that MAR profiles of E. coli may only be marginally successful in identifying sources of faecal pollution. Significance and Impact of the Study: This study demonstrates the existence of regional and seasonal differences in MAR profiles as well as the limited ability of MAR profiles to discriminate among livestock sources.Keywords
This publication has 10 references indexed in Scilit:
- Use of Antibiotic Resistance Analysis for Representativeness Testing of Multiwatershed LibrariesApplied and Environmental Microbiology, 2003
- Using Multiple Antibiotic Resistance and Land use Characteristics to Determine Sources of Fecal Coliform Bacterial PollutiionEnvironmental Monitoring and Assessment, 2003
- Advances in the Bacteriology of the Coliform Group: Their Suitability as Markers of Microbial Water SafetyAnnual Review of Microbiology, 2001
- Classification of Antibiotic Resistance Patterns of Indicator Bacteria by Discriminant Analysis: Use in Predicting the Source of Fecal Contamination in Subtropical WatersApplied and Environmental Microbiology, 2000
- Protection of Water Quality:Journal of Sustainable Agriculture, 1996
- Microtitration plate most-probable-number tests for the enumeration of Escherichia coli in estuarine and marine watersJournal of Microbiological Methods, 1993
- Discriminant Analysis and Statistical Pattern RecognitionWiley Series in Probability and Statistics, 1992
- Antibiotic resistance indexing of Escherichia coli to identify sources of fecal contamination in waterCanadian Journal of Microbiology, 1990
- Effect of disinfectants on survival of cryptosporidium oocystsVeterinary Record, 1982
- Antibiotic Resistance Among Coliform and Fecal Coliform Bacteria Isolated from Sewage, Seawater, and Marine ShellfishAntimicrobial Agents and Chemotherapy, 1976