Incidence of cryptosporidiosis species in paediatric patients in Malawi
- 15 January 2007
- journal article
- Published by Cambridge University Press (CUP) in Epidemiology and Infection
- Vol. 135 (8), 1307-1315
- https://doi.org/10.1017/s0950268806007758
Abstract
SUMMARY: We determined the incidence of cryptosporidiosis in children aged Cryptosporidiumspp. oocysts. Species determination was by polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) of oocyst-extracted DNA using 18S rRNA and COWP gene loci.Cryptosporidiumspp. oocysts were seen in 5·9% (50/848) of samples, of which 43 amplified by PCR–RFLP indicated the following species:C. hominis,C. parvum,C. hominis/C. parvum,C. meleagridisandC. andersoni. Seven samples could not be amplified by PCR. Wider species diversity was found in the rural setting, and may be a result of increased malnutrition and zoonotic exposure in this area. Improvements in water, sanitation, household hygiene and animal control are required to reduce the incidence of infection in this population.This publication has 44 references indexed in Scilit:
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