Decline in intestinal mucosal IL-10 expression and decreased intestinal barrier function in a mouse model of total parenteral nutrition
Open Access
- 1 January 2008
- journal article
- Published by American Physiological Society in American Journal of Physiology-Gastrointestinal and Liver Physiology
- Vol. 294 (1), G139-G147
- https://doi.org/10.1152/ajpgi.00386.2007
Abstract
Loss of intestinal epithelial barrier function (EBF) is a major problem associated with total parenteral nutrition (TPN) administration. We have previously identified intestinal intraepithelial lymphocyte (IEL)-derived interferon-γ (IFN-γ) as a contributing factor to this barrier loss. The objective was to determine whether other IEL-derived cytokines may also contribute to intestinal epithelial barrier breakdown. C57BL6J male mice received TPN or enteral nutrition (control) for 7 days. IEL-derived interleukin-10 (IL-10) was then measured. A significant decline in IEL-derived IL-10 expression was seen with TPN administration, a cytokine that has been shown in vitro to maintain tight junction integrity. We hypothesized that this change in IEL-derived IL-10 expression could contribute to TPN-associated barrier loss. An additional group of mice was given exogenous recombinant IL-10. Ussing chamber experiments showed that EBF markedly declined in the TPN group. TPN resulted in a significant decrease of IEL-derived IL-10 expression. The expression of several tight junction molecules also decreased with TPN administration. Exogenous IL-10 administration in TPN mice significantly attenuated the TPN-associated decline in zonula occludens (ZO)-1, E-cadherin, and occludin expression, as well as a loss of intestinal barrier function. TPN administration led to a marked decline in IEL-derived IL-10 expression. This decline was coincident with a loss of intestinal EBF. As the decline was partially attenuated with the administration of exogenous IL-10, our findings suggest that loss of IL-10 may be a contributing mechanism to TPN-associated epithelial barrier loss.Keywords
This publication has 79 references indexed in Scilit:
- Repertoire of the αβ T‐cell receptor in the intestineImmunological Reviews, 2007
- Molecular Basis of Epithelial Barrier Regulation: From Basic Mechanisms to Clinical ApplicationThe American Journal of Pathology, 2006
- Different Changes in the Expression of Multiple Kinds of Tight-Junction Proteins during Ischemia-Reperfusion Injury of the Rat IleumACTA HISTOCHEMICA ET CYTOCHEMICA, 2006
- Mucosal integrity and barrier function in the pathogenesis of early lesions in Crohn's diseaseJournal of Clinical Pathology, 2005
- TROPHIC AND CYTOPROTECTIVE NUTRITION FOR INTESTINAL ADAPTATION, MUCOSAL REPAIR, AND BARRIER FUNCTIONAnnual Review of Nutrition, 2003
- Intestinal barrier functionCurrent Opinion in Clinical Nutrition and Metabolic Care, 2002
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- Beneficial effects of insulin-like growth factor I on epithelial structure and function in parenterally fed rat jejunumGastroenterology, 1996
- The association of gut-associated lymphoid tissue and bacterial translocation in the newborn rabbitJournal of Pediatric Surgery, 1996
- Enterocolitis and colon cancer in interleukin-10-deficient mice are associated with aberrant cytokine production and CD4(+) TH1-like responses.JCI Insight, 1996