Effect of D2O in vivo and in vitro on uptake of p-aminohippurate by rat kidney slices

Abstract

Kidney slices from rats in which one-third of the body water was replaced by D₂O showed no impairment of the capacity to accumulate PAH, despite the moribund condition of the animals. In vitro, 100% D₂O inhibited PAH uptake by 65%, oxygen consumption by only 15%, during a 1-hour incubation of kidney slices; very little PAH was taken up after the first 30 minutes.