The bZIP Transcription Factor MeaB Mediates Nitrogen Metabolite Repression at Specific Loci
- 1 October 2010
- journal article
- research article
- Published by American Society for Microbiology in Eukaryotic Cell
- Vol. 9 (10), 1588-1601
- https://doi.org/10.1128/ec.00146-10
Abstract
In Fusarium fujikuroi , bikaverin (BIK) biosynthesis is subject to repression by nitrogen. Unlike most genes subject to nitrogen metabolite repression, it has been shown that transcription of bik biosynthetic genes is not AreA dependent. Searching for additional transcription factors that may be involved in nitrogen regulation, we cloned and characterized the orthologue of Aspergillus nidulans meaB , which encodes a bZIP transcription factor. Two transcripts are derived from F. fujikuroi meaB : the large transcript ( meaB L ) predominates under nitrogen-sufficient conditions and the smaller transcript ( meaB S ) under nitrogen limitation, in an AreA-dependent manner. MeaB is specifically translocated to the nucleus under nitrogen-sufficient conditions in both F. fujikuroi and A. nidulans . Deletion of meaB resulted in partial upregulation of several nitrogen-regulated genes, but only in the Δ meaB Δ areA double mutant were the bikaverin genes significantly upregulated in the presence of glutamine. These data demonstrate that MeaB and AreA coordinately mediate nitrogen metabolite repression and, importantly, that independently of AreA, MeaB can mediate nitrogen metabolite repression at specific loci in F. fujikuroi .Keywords
This publication has 49 references indexed in Scilit:
- FfVel1 and FfLae1, components of a velvet‐like complex in Fusarium fujikuroi, affect differentiation, secondary metabolism and virulenceMolecular Microbiology, 2010
- Identification of virulence genes in Fusarium oxysporum f. sp. lycopersici by large‐scale transposon taggingMolecular Plant Pathology, 2009
- Cross-Species Hybridization with Fusarium verticillioides Microarrays Reveals New Insights into Fusarium fujikuroi Nitrogen Regulation and the Role of AreA and NMREukaryotic Cell, 2008
- Impact of Ammonium Permeases MepA, MepB, and MepC on Nitrogen-Regulated Secondary Metabolism in Fusarium fujikuroiEukaryotic Cell, 2008
- Transcriptional control of nmrA by the bZIP transcription factor MeaB reveals a new level of nitrogen regulation in Aspergillus nidulansMolecular Microbiology, 2007
- Role of the Fusarium fujikuroi TOR Kinase in Nitrogen Regulation and Secondary MetabolismEukaryotic Cell, 2006
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- Molecular characterisation of meaB, a novel gene affecting nitrogen metabolite repression in Aspergillus nidulansFEBS Letters, 1996
- Chloroplast DNA Phylogenetic Affinities of Newly Described Species in Glycine (Leguminosae: Phaseoleae)Systematic Botany, 1990
- Isolation and characterization of the glyceraldehyde-3-phosphate dehydrogenase gene of Aspergillus nidulansGene, 1988