Three-dimensional ultrastructural analysis of the Saccharomyces cerevisiae mitotic spindle.
Open Access
- 15 June 1995
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 129 (6), 1601-1615
- https://doi.org/10.1083/jcb.129.6.1601
Abstract
The three dimensional organization of microtubules in mitotic spindles of the yeast Saccharomyces cerevisiae has been determined by computer-aided reconstruction from electron micrographs of serially cross-sectioned spindles. Fifteen spindles ranging in length from 0.6-9.4 microns have been analyzed. Ordered microtubule packing is absent in spindles up to 0.8 micron, but the total number of microtubules is sufficient to allow one microtubule per kinetochore with a few additional microtubules that may form an interpolar spindle. An obvious bundle of about eight interpolar microtubules was found in spindles 1.3-1.6 microns long, and we suggest that the approximately 32 remaining microtubules act as kinetochore fibers. The relative lengths of the microtubules in these spindles suggest that they may be in an early stage of anaphase, even though these spindles are all situated in the mother cell, not in the isthmus between mother and bud. None of the reconstructed spindles exhibited the uniform populations of kinetochore microtubules characteristic of metaphase. Long spindles (2.7-9.4 microns), presumably in anaphase B, contained short remnants of a few presumed kinetochore microtubules clustered near the poles and a few long microtubules extending from each pole toward the spindle midplane, where they interdigitated with their counterparts from the other pole. Interpretation of these reconstructed spindles offers some insights into the mechanisms of mitosis in this yeast.Keywords
This publication has 22 references indexed in Scilit:
- Interpolar spindle microtubules in PTK cells.The Journal of cell biology, 1993
- NDC10: a gene involved in chromosome segregation in Saccharomyces cerevisiae.The Journal of cell biology, 1993
- The centromere frontier: Kinetochore components, microtubule-based motility, and the CEN-value paradoxCell, 1993
- Three-dimensional reconstruction and analysis of mitotic spindles from the yeast, Schizosaccharomyces pombe.The Journal of cell biology, 1993
- Astral microtubules are not required for anaphase B in Saccharomyces cerevisiae.The Journal of cell biology, 1992
- Visualization of centromeric and nucleolar DNA in fission yeast by fluorescence in situ hybridizationJournal of Cell Science, 1992
- Routine cryofixation of plant tissue by propane jet freezing for freeze substitutionJournal of Electron Microscopy Technique, 1991
- Electron-microscopic study of the spindle and chromosome movement in the yeast Saccharomyces CerevisiaeJournal of Cell Science, 1976
- Electron microscopic observations on the meiotic karyotype of diploid and tetraploid Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America, 1975
- Duplication of Spindle Plaques and Integration of the Yeast Cell CyclePublished by Cold Spring Harbor Laboratory ,1974