Pseudotyped Influenza A Virus as a Vaccine for the Induction of Heterotypic Immunity
- 15 December 2012
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 86 (24), 13397-13406
- https://doi.org/10.1128/jvi.01820-12
Abstract
There is a need for vaccines that can protect broadly across all influenza A strains. We have produced a pseudotyped influenza virus based on suppression of the A/PR/8/34 hemagglutinin signal sequence (S-FLU) that can infect cells and express the viral core proteins and neuraminidase but cannot replicate. We show that when given by inhalation to mice, S-FLU is nonpathogenic but generates a vigorous T cell response in the lung associated with markedly reduced viral titers and weight loss after challenge with H1 and H3 influenza viruses. These properties of S-FLU suggest that it may have potential as a broadly protective A virus vaccine, particularly in the setting of a threatened pandemic before matched subunit vaccines become available.Keywords
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