Determination of Explosives and Some Metabolites of TNT in Biological and Environmental Samples by Liquid Chromatography on a Mixed-Mode C18-anion Column
- 1 February 1996
- journal article
- research article
- Published by Taylor & Francis Ltd in Journal of Liquid Chromatography & Related Technologies
- Vol. 19 (4), 661-677
- https://doi.org/10.1080/10826079608005527
Abstract
This method employs a ternary gradient generated from (1) a 90:10 water:methanol solution that is 0.015 M in potassium phosphate at pH 5.1; (2) methanol; and (3) acetonitrile to separate fifteen explosives, byproducts, and metabolites of 2,4,6-trinitrotoluene (TNT) on a C18/anion exchange stationary phase. TNT, hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX), six reduction metabolites and three oxidation metabolites of TNT have been separated and quantitated in a single chromatographic run. Because of the anion exchange character of the stationary phase, the anionic TNT metabolite 2,4,6-trinitrobenzoic acid also may be separated and quantitated using this method. Quantitation limits generally lower than 1 ppm have been established by a systematic certification procedure. The performance of the mixed mode separation method combined with diode array ultraviolet absorbance detection has been demonstrated with samples derived from both biological (tissues and physiological fluids) and environmental (soil, composts, and leachates) sources.Keywords
This publication has 29 references indexed in Scilit:
- Use of narrow-bore high-performance liquid chromatography-diode array detection for the analysis of intermediates of the biological degradation of 2,4,6-trinitrotolueneJournal of Chromatography A, 1995
- Determination of Explosives and Their Biodegradation Products in Contaminated Soil and Water from Former Ammunition Plants by Automated Multiple Development High-Performance Thin-Layer ChromatographyAnalytical Chemistry, 1994
- Determination of RDX, 2,4,6-trinitrotoluene and other nitroaromatic compounds by high-performance liquid chromatography with photodiode- array detectionJournal of Chromatography A, 1993
- Determination of explosives residues in soils by micellar electrokinetic capillary chromatography and high-performance liquid chromatography: A comparative studyJournal of Chromatography A, 1993
- Liquid chromatographic separation of 2,4,6-trinitrotoluene and its principal reduction productsAnalytica Chimica Acta, 1990
- Applications of liquid chromatography—mass spectrometry in metabolic studies of explosivesJournal of Chromatography A, 1987
- Trace analysis of explosives by thermospray high-performance liquid chromatography—mass spectrometryJournal of Chromatography A, 1986
- High-performance liquid chromatography—mass spectrometry of explosivesJournal of Chromatography A, 1983
- Separation of mixtures of 2,4,6-trinitrotoluene reduction products with liquid chromatographyAnalytica Chimica Acta, 1982
- Effluent analysis of wastewater generated in the manufacture of 2,4,6-trinitrotoluene. 1. Characterization studyEnvironmental Science & Technology, 1982