A 12 megabase restriction map at the cystic fibrosis locus
Open Access
- 11 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 17 (1), 271-284
- https://doi.org/10.1093/nar/17.1.271
Abstract
We have constructed a physical map of the chromosomal region containing the cystic fibrosis locus using seven DNA markers and pulsed-field gel electrophoresis methods. The map includes cleavage sites for 8 rare-cutting restriction enzymes and spans over 12 megabases (Mb) of DNA, with one unlinked probe covering an additional 5 Mb. To our knowledge, this is the largest segment of human DNA which has been restriction mapped to date. We can identify thirteen putative HTF islands spaced at intervals of 0.3 − 3.2 Mb. The region between loci D7S8 and MET, where the CF gene lies, includes 1.4 − 1.9 Mb of DNA.Keywords
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