Protein Engineering for Cardiovascular Therapeutics
- 13 September 2013
- journal article
- review article
- Published by Ovid Technologies (Wolters Kluwer Health) in Circulation Research
- Vol. 113 (7), 933-943
- https://doi.org/10.1161/circresaha.113.300215
Abstract
A number of new and innovative approaches for repairing damaged myocardium are currently undergoing investigation, with several encouraging results. In addition to the progression of stem cell–based approaches and gene therapy/silencing methods, evidence continues to emerge that protein therapeutics may be used to directly promote cardiac repair and even regeneration. However, proteins are often limited in their therapeutic potential by short local half-lives and insufficient bioavailability and bioactivity, and many academic laboratories studying cardiovascular diseases are more comfortable with molecular and cellular biology than with protein biochemistry. Protein engineering has been used broadly to overcome weaknesses traditionally associated with protein therapeutics and has the potential to specifically enhance the efficacy of molecules for cardiac repair. However, protein engineering as a strategy has not yet been used in the development of cardiovascular therapeutics to the degree that it has been used in other fields. In this review, we discuss the role of engineered proteins in cardiovascular therapies to date. Further, we address the promise of applying emerging protein engineering technologies to cardiovascular medicine and the barriers that must be overcome to enable the ultimate success of this approach.Keywords
This publication has 153 references indexed in Scilit:
- Cardiac RegenerationCurrent Topics in Developmental Biology, 2012
- Cardiac stem cells in patients with ischaemic cardiomyopathy (SCIPIO): initial results of a randomised phase 1 trialThe Lancet, 2011
- Regeneration of the heartEMBO Molecular Medicine, 2011
- Intravenous Erythropoietin in Patients With ST-Segment Elevation Myocardial InfarctionJama-Journal Of The American Medical Association, 2011
- Paracrine mechanisms of stem cell reparative and regenerative actions in the heartJournal of Molecular and Cellular Cardiology, 2011
- Engineered Cystine-Knot Peptides that Bind αvβ3 Integrin with Antibody-Like AffinitiesJournal of Molecular Biology, 2009
- Design, Synthesis, and Actions of a Novel Chimeric Natriuretic Peptide: CD-NPJournal of the American College of Cardiology, 2008
- Engineering novel binding proteins from nonimmunoglobulin domainsNature Biotechnology, 2005
- The biology of VEGF and its receptorsNature Medicine, 2003
- Transduction of full-length TAT fusion proteins into mammalian cells: TAT-p27Kip1 induces cell migrationNature Medicine, 1998