A validated stability indicating HPTLC method for simultaneous estimation of irbesartan and hydrochlorothiazide
Open Access
- 1 January 2010
- journal article
- research article
- Published by EManuscript Technologies in Pharmaceutical Methods
- Vol. 1 (1), 39-43
- https://doi.org/10.4103/2229-4708.72229
Abstract
Introduction: Irbesartan, a diazaspiro angiotensin II blocker, is marketed in combination with Hydrochlorothiazide, which is a diuretic acting on distal convoluted tubule; for synergistic anti-hypertensive action. The present study deals with development and validation of a stability indicating HPTLC method for simultaneous estimation of Irbesartan and Hydrochlorothiazide using TLC plates precoated with Silica gel 60F254 and the mobile phase comprising Acetonitrile: Chloroform in the ratio of 5:6 v/v. Irbesartan and Hydrochlorothiazide were well resolved with Rf 0.27 ± 0.03 and 0.45 ± 0.03, respectively. Wavelength selected for the quantization was 270 nm. Inherent stability of these drugs was studied by exposing both drugs to various stress conditions as per ICH guidelines viz. Dry heat, oxidative, photolysis (UV and cool white fluorescent light) and hydrolytic conditions under different pH values. Results: Both the drugs were not degraded under dry heat and photolytic conditions, but showed degradation under hydrolytic condition. The degraded products of Irbesartan and hydrochlorothiazide were well resolved from the individual bulk drug response. Conclusion: The developed method is found to be simple, specific, precise and stability indicating. The specificity of the method was confirmed by peak purity profile of the resolved peaks.Keywords
This publication has 7 references indexed in Scilit:
- Simultaneous determination of irbesartan and hydrochlorothiazide in human plasma using HPLC coupled with tandem mass spectrometry: Application to bioequivalence studiesJournal of Pharmaceutical and Biomedical Analysis, 2010
- HPTLC method for the simultaneous estimation of valsartan and hydrochlorothiazide in tablet dosage formIndian Journal of Pharmaceutical Sciences, 2009
- Simultaneous determination of irbesartan and hydrochlorothiazide in human plasma by liquid chromatographyJournal of Chromatography B, 2002
- Fast screening method for the determination of angiotensin II receptor antagonists in human plasma by high-performance liquid chromatography with fluorimetric detectionJournal of Chromatography A, 2001
- Spectrophotometric and HPTLC-densitometric determination of lisinopril and hydrochlorothiazide in binary mixturesJournal of Pharmaceutical and Biomedical Analysis, 2001
- High-performance liquid chromatographic assay for the quantitation of irbesartan (SR 47436/BMS-186295) in human plasma and urineJournal of Chromatography B: Biomedical Sciences and Applications, 1997
- Stability-Indicating Assay for HydrochlorothiazideJournal of Pharmaceutical Sciences, 1981