End-functionalized glycopolymers as mimetics of chondroitin sulfate proteoglycans
Open Access
- 23 July 2010
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Chemical Science
- Vol. 1 (3), 322-325
- https://doi.org/10.1039/c0sc00271b
Abstract
Glycosaminoglycans are sulfated polysaccharides that play important roles in fundamental biological processes, such as cell division, viral invasion, cancer and neuroregeneration. The multivalent presentation of multiple glycosaminoglycan chains on proteoglycan scaffolds may profoundly influence their interactions with proteins and subsequent biological activity. However, the importance of this multivalent architecture remains largely unexplored, and few synthetic mimics exist for probing and manipulating glycosaminoglycan activity. Here, we describe a new class of end-functionalized ring-opening metathesis polymerization (ROMP) polymers that mimic the native-like, multivalent architecture found on chondroitin sulfate (CS) proteoglycans. We demonstrate that these glycopolymers can be readily integrated with microarray and surface plasmon resonance technology platforms, where they retain the ability to interact selectively with proteins. ROMP-based glycopolymers are part of a growing arsenal of chemical tools for probing the functions of glycosaminoglycans and for studying their interactions with proteins.This publication has 35 references indexed in Scilit:
- PTPσ Is a Receptor for Chondroitin Sulfate Proteoglycan, an Inhibitor of Neural RegenerationScience, 2009
- Profiling the Sulfation Specificities of Glycosaminoglycan Interactions with Growth Factors and Chemotactic Proteins Using MicroarraysCell Chemical Biology, 2007
- Discovery of a TNF-α Antagonist Using Chondroitin Sulfate MicroarraysJournal of the American Chemical Society, 2006
- Chemical approaches to deciphering the glycosaminoglycan codeCurrent Opinion in Chemical Biology, 2005
- Structural Insights into Biological Roles of Protein-Glycosaminoglycan InteractionsCell Chemical Biology, 2005
- Novel 70-kDa Chondroitin Sulfate/Dermatan Sulfate Hybrid Chains with a Unique Heterogenous Sulfation Pattern from Shark Skin, Which Exhibit Neuritogenic Activity and Binding Activities for Growth Factors and Neurotrophic FactorsPublished by Elsevier BV ,2005
- Chondroitin proteoglycans are involved in cell division of Caenorhabditis elegansNature, 2003
- Specific Molecular Interactions of Oversulfated Chondroitin Sulfate E with Various Heparin-binding Growth FactorsPublished by Elsevier BV ,2002
- Heparin-Protein InteractionsAngewandte Chemie-International Edition, 2002
- Distribution of iduronate 2-sulphate residues in heparan sulphate. Evidence for an ordered polymeric structureBiochemical Journal, 1991