Enhanced Reductive Dechlorination of Polychlorinated Biphenyl Impacted Sediment by Bioaugmentation with a Dehalorespiring Bacterium
- 8 September 2011
- journal article
- research article
- Published by American Chemical Society (ACS) in Environmental Science & Technology
- Vol. 45 (20), 8772-8779
- https://doi.org/10.1021/es201553c
Abstract
Anaerobic reductive dehalogenation of commercial PCBs such as Aroclor 1260 has a critical role of transforming highly chlorinated congeners to less chlorinated congeners that are then susceptible to aerobic degradation. The efficacy of bioaugmentation with the dehalorespiring bacterium Dehalobium chlorocoercia DF1 was tested in 2-L laboratory mesocosms containing sediment contaminated with weathered Aroclor 1260 (1.3 ppm) from Baltimore Harbor, MD. Total penta- and higher chlorinated PCBs decreased by approximately 56% (by mass) in bioaugmented mesocosms after 120 days compared with no activity observed in unamended controls. Bioaugmentation with DF-1 enhanced the dechlorination of doubly flanked chlorines and stimulated the dechlorination of single flanked chlorines as a result of an apparent synergistic effect on the indigenous population. Addition of granulated activated carbon had a slight stimulatory effect indicating that anaerobic reductive dechlorination of PCBs at low concentrations was not inhibited by a high background of inorganic carbon that could affect bioavailability. The total number of dehalorespiring bacteria was reduced by approximately half after 60 days. However, a steady state level was maintained that was greater than the indigenous population of putative dehalorespiring bacteria in untreated sediments and DF1 was maintained within the indigenous population after 120 days. The results of this study demonstrate that bioaugmentation with dehalorespiring bacteria has a stimulatory effect on the dechlorination of weathered PCBs and supports the feasibility of using in situ bioaugmentation as an environmentally less invasive and lower cost alternate to dredging for treatment of PCB impacted sediments.Keywords
This publication has 41 references indexed in Scilit:
- In-situ Sorbent Amendments: A New Direction in Contaminated Sediment ManagementPublished by American Chemical Society (ACS) ,2011
- “ Dehalococcoides ” sp. Strain CBDB1 Extensively Dechlorinates the Commercial Polychlorinated Biphenyl Mixture Aroclor 1260Applied and Environmental Microbiology, 2009
- Dehalorespiration with Polychlorinated Biphenyls by an Anaerobic UltramicrobacteriumApplied and Environmental Microbiology, 2008
- Microbial Reductive Dechlorination of Aroclor 1260 in Baltimore Harbor Sediment Microcosms Is Catalyzed by Three Phylotypes within the Phylum ChloroflexiApplied and Environmental Microbiology, 2007
- The Dehalococcoides Population in Sediment-Free Mixed Cultures Metabolically Dechlorinates the Commercial Polychlorinated Biphenyl Mixture Aroclor 1260Applied and Environmental Microbiology, 2007
- Identification of a Bacterium That Specifically Catalyzes the Reductive Dechlorination of Polychlorinated Biphenyls with Doubly Flanked ChlorinesApplied and Environmental Microbiology, 2002
- Use of Halogenated Benzoates and Other Halogenated Aromatic Compounds To Stimulate the Microbial Dechlorination of PCBsEnvironmental Science & Technology, 1999
- Enrichment of Microorganisms That Sequentially meta, para-Dechlorin- ate the Residue of Aroclor 1260 in Housatonic River SedimentEnvironmental Science & Technology, 1997
- High-resolution PCB analysis: synthesis and chromatographic properties of all 209 PCB congenersEnvironmental Science & Technology, 1984
- An improved preparation method for a titanium (III) media reductantJournal of Microbiological Methods, 1983