Deposition of bismuth by Yersinia enterocolitica
- 1 July 1992
- journal article
- research article
- Published by Springer Science and Business Media LLC in Medical Microbiology and Immunology
- Vol. 181 (3), 145-152
- https://doi.org/10.1007/bf00202054
Abstract
Yersinia enterocolitica 808lc cultures in exponential growth were incubated for 1 h in 0.1 % microcrystalline bismuth subsalicylate (BSS) suspensions. Scanning electron microscopy (SEM) revealed microcrystals directly bound to BSS-treated bacteria. Energy dispersive spectroscopy (EDS) X-ray microanalysis of the attached microcrystals confirmed that the crystals were the microcrystalline BSS. X-ray spectra positive for bismuth were also obtained by SEM-EDS X-ray microanalysis of whole bacteria, suggesting metal incorporation into the bacteria in regions absent of bound microcrystals. Transmission electron microscopy of thin sections of embedded preparations of BSS-treated exponential-growth-phase bacteria showed electron-dense deposits in the periphery of the bacteria. Y. enterocolitica cultures that were in stationary phase at the time of incubation with microcrystalline BSS showed no evidence of the electron-dense deposits and EDS spectra were negative for bismuth. Bacteria incubated in the absence of microcrystalline BSS also lacked electron-dense deposits. Scanning transmission electron microscopy used in conjunction with EDS X-ray microanalysis to view and analyze semi-thick sections (250–300 nm) of embedded preparations of BSS-treated bacteria in exponential growth confirmed that the electron-dense deposits at the periphery of the bacteria are the sites of bismuth depositions.Keywords
This publication has 11 references indexed in Scilit:
- In Vitro Antibacterial Activity of Bismuth SubsalicylateClinical Infectious Diseases, 1990
- In Vitro Antimicrobial Activity of Bismuth Subsalicylate and Other Bismuth SaltsClinical Infectious Diseases, 1990
- Binding and killing of bacteria by bismuth subsalicylateAntimicrobial Agents and Chemotherapy, 1989
- Microbial metabolism, desolubilization, and deposition of heavy metals: metal uptake by immobilized cells and application to the detoxification of liquid wastes.1989
- Plasmid-Mediated Heavy Metal ResistancesAnnual Review of Microbiology, 1988
- Response of Campylobacter pyloridis to antibiotics, bismuth and an acid-reducing agent in vitro--an ultrastructural studyJournal of Medical Microbiology, 1987
- [Electron microscopic findings during treatment of Campylobacter pylori-positive gastritis with bismuth salts].1987
- Antibacterial Action of Bismuth in Relation to Campylobacter pyloridis Colonization and GastritisDigestion, 1987
- Microorganisms in Reclamation of MetalsAnnual Review of Microbiology, 1986
- Reduction of inorganic compounds with molecular hydrogen by Micrococcus lactilyticus. I. Stoichiometry with compounds of arsenic, selenium, tellurium, transition and other elements.1962