Monoclonal Antibodies against the Candidate Lupin Allergens α-Conglutin and β-Conglutin
- 28 December 2006
- journal article
- Published by S. Karger AG in International Archives of Allergy and Immunology
- Vol. 143 (1), 49-58
- https://doi.org/10.1159/000098224
Abstract
The ingestion of dietary products containing sweet lupin (such as Lupinus albus or Lupinus angustifolius) has been reported to cause IgE-mediated allergic reactions. Recent studies have indicated lupin globulins as important IgE binding proteins. The aim of the present study was to generate and characterize monoclonal antibodies (mAbs) against lupin seed proteins. Mice were immunized with a protein isolate from L. albus and mAbs were obtained by hybridoma techniques. Albumins and globulins were extracted, and the globulin fraction was separated further into conglutins by anion exchange chromatography. Specificities, binding patterns and applications of the mAbs were investigated by immunochemical methods. Five mAbs were produced: Lu11 (an IgG2b antibody), Lu8, Lu18, Lu34 and Lu35 (all IgM antibodies). The mAbs reacted strongly with protein isolates from both L. albus and L. angustifolius. All mAbs are directed towards the lupin globulin fraction; Lu11 and Lu18 recognize alpha-conglutin, while Lu8, Lu34 and Lu35 recognize beta-conglutin. In addition, Lu11 inhibited the binding of IgE from patients with positive skin prick tests to lupin proteins in a competitive ELISA by approximately 30%. Furthermore, preliminary results show that Lu11 can be used to develop a sensitive method for the detection of alpha-conglutin in foods. Lupin globulins are immunogenic and alpha-conglutin is a potential allergen. This is the first study describing mAbs against the candidate lupin allergens, emphasizing the importance of additional studies on conglutins in lupin allergy.Keywords
This publication has 32 references indexed in Scilit:
- Peanut allergenicityAnnals of Allergy, Asthma & Immunology, 2004
- Vicilin and convicilin are potential major allergens from peaClinical and Experimental Allergy, 2004
- Allergy to lupine flourAllergy, 2001
- Lupine-induced anaphylaxisAnnals of Allergy, Asthma & Immunology, 1999
- Contact urticaria form lupinContact Dermatitis, 1997
- Absorption of zinc from Iupin (Lupinus angustifolius)-based foodsBritish Journal of Nutrition, 1994
- The seed storage proteins from Lupinus albusPhytochemistry, 1994
- Heat‐induced synthesis and tunicamycin‐sensitive secretion of the putative storage glycoprotein conglutin γ from mature lupin seedsEuropean Journal of Biochemistry, 1994
- Sensory Evaluation and Acceptability of Cookies Enriched with Sweet Lupine Flour (Lupinus albus cv Multolupa)Journal of Food Science, 1987
- Subunit composition of the seed globulins of Lupinus albusPhytochemistry, 1981