From pull-down data to protein interaction networks and complexes with biological relevance

Abstract

Motivation: Recent improvements in high-throughput Mass Spectrometry (MS) technology have expedited genome-wide discovery of protein–protein interactions by providing a capability of detecting protein complexes in a physiological setting. Computational inference of protein interaction networks and protein complexes from MS data are challenging. Advances are required in developing robust and seamlessly integrated procedures for assessment of protein–protein interaction affinities, mathematical representation of protein interaction networks, discovery of protein complexes and evaluation of their biological relevance.