Angiogenic activity of human chorionic gonadotropin through LH receptor activation on endothelial and epithelial cells of the endometrium
- 25 October 2006
- journal article
- Published by Wiley in The FASEB Journal
- Vol. 20 (14), 2630-2632
- https://doi.org/10.1096/fj.06-5885fje
Abstract
Successful embryo development requires an extensive endometrial angiogenesis in proximity of implantation site. The glycoprotein hCG is produced even before implantation by trophoblast in normal pregnancy. In this manuscript, we demonstrate an angiogenic effect of hCG in several in vivo (chick chorioallantoïc membrane, matrigel plug assay, aortic ring assay) and in vitro experimental models. In contrast, human placental lactogen (hPL) did not display angiogenic properties. LH/hCG receptor was detected in endothelial cells by reverse-transcriptase polymerase chain reaction (RT-PCR) and by Western blotting. In mice aortic ring assay, angiostimulation by hCG was abrogated by deletion of LH/hCG receptor (LuRKO mice). Use of recombinant hCG and anti–hCG antibody (Ab) further confirmed the specificity of this angiogenic activity. By using dibutyryl cAMP, adenylate cyclase, or protein kinase A inhibitors, we demonstrate that hCG-mediated angiogenesis involves adenylyl-cyclase–protein kinase A activation. Addition of hCG to endometrial epithelial epithelial cells, but not to cultured endothelial cells, stimulated vascular endothelial growth factor (VEGF). VEGF and hCG also displayed additive activities. Altogether, these data demonstrate that peritrophoblastic angiostimulation may result from a paracrine dialogue between trophoblast, epithelial, and endothelial cells through hCG and VEGF.—Berndt, S., Perrier d’Hauterive, S., Blacher, S., Péqueux, C., Lorquet, S., Munaut, C., Applanat, M., Hervé, M. A., Lamandé, N., Corvol, P., van den Brûle, F., Frankenne, F., Poutanen, M., Huhtaniemi, I., Geenen, V., Noël, A., Foidart, J.-M. Angiogenic activity of human chorionic gonadotropin through LH receptor activation on endothelial and epithelial cells of the endometrium.Keywords
This publication has 45 references indexed in Scilit:
- In Vivo Infusion of Interleukin-1β and Chorionic Gonadotropin Induces Endometrial Changes that Mimic Early Pregnancy Events in the BaboonEndocrinology, 2005
- Prevention of Endometrial Apoptosis: Randomized Prospective Comparison of Human Chorionic GonadotropinVersusProgesterone Treatment in the Luteal PhaseJournal of Clinical Endocrinology & Metabolism, 2005
- Human chorionic gonadotropin and growth factors at the embryonic–endometrial interface control leukemia inhibitory factor (LIF) and interleukin 6 (IL-6) secretion by human endometrial epitheliumHuman Reproduction, 2004
- Chorionic gonadotropin and uterine dialogue in the primateReproductive Biology and Endocrinology, 2004
- Human chorionic gonadotropin/luteinizing hormone receptor expression in the adult rat spinal cordNeuroscience Letters, 2002
- Ultrastructure of epithelial plaque formation and stromal cell transformation by post-ovulatory chorionic gonadotrophin treatment in the baboon (Papio anubis)Human Reproduction, 2001
- Cytokine control in human endometriumReproduction, 2001
- Implantation in the Baboon: Endometrial ResponsesSeminars in Reproductive Medicine, 1999
- Localization of receptors for luteinizing hormone/chorionic gonadotropin in neural retinaLife Sciences, 1998
- The presence of luteinizing hormone/human chorionic gonadotropin receptors in lactating rat mammary glandsLife Sciences, 1997