Kinetic and Spectral Resolution of Multiple Nonphotochemical Quenching Components in Arabidopsis Leaves
- 23 December 2009
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 152 (3), 1611-1624
- https://doi.org/10.1104/pp.109.148213
Abstract
Using novel specially designed instrumentation, fluorescence emission spectra were recorded from Arabidopsis (Arabidopsis thaliana) leaves during the induction period of dark to high-light adaptation in order to follow the spectral changes associated with the formation of nonphotochemical quenching. In addition to an overall decrease of photosystem II fluorescence (quenching) across the entire spectrum, high light induced two specific relative changes in the spectra: (1) a decrease of the main emission band at 682 nm relative to the far-red (750–760 nm) part of the spectrum (Δ F682); and (2) an increase at 720 to 730 nm (Δ F720) relative to 750 to 760 nm. The kinetics of the two relative spectral changes and their dependence on various mutants revealed that they do not originate from the same process but rather from at least two independent processes. The Δ F720 change is specifically associated with the rapidly reversible energy-dependent quenching. Comparison of the wild-type Arabidopsis with mutants unable to produce or overexpressing the PsbS subunit of photosystem II showed that PsbS was a necessary component for Δ F720. The spectral change Δ F682 is induced both by energy-dependent quenching and by PsbS-independent mechanism(s). A third novel quenching process, independent from both PsbS and zeaxanthin, is activated by a high turnover rate of photosystem II. Its induction and relaxation occur on a time scale of a few minutes. Analysis of the spectral inhomogeneity of nonphotochemical quenching allows extraction of mechanistically valuable information from the fluorescence induction kinetics when registered in a spectrally resolved fashion.Keywords
This publication has 81 references indexed in Scilit:
- Lutein Accumulation in the Absence of Zeaxanthin Restores Nonphotochemical Quenching in the Arabidopsis thaliana npq1 MutantPlant Cell, 2009
- Lutein Can Act as a Switchable Charge Transfer Quencher in the CP26 Light-harvesting ComplexJournal of Biological Chemistry, 2009
- The Zeaxanthin-Independent and Zeaxanthin-Dependent qE Components of Nonphotochemical Quenching Involve Common Conformational Changes within the Photosystem II Antenna in ArabidopsisPlant Physiology, 2008
- Far-red fluorescence: A direct spectroscopic marker for LHCII oligomer formation in non-photochemical quenchingFEBS Letters, 2008
- Trap-Limited Charge Separation Kinetics in Higher Plant Photosystem I ComplexesBiophysical Journal, 2008
- Control of the light harvesting function of chloroplast membranes: The LHCII‐aggregation model for non‐photochemical quenchingFEBS Letters, 2005
- Photoinhibition of Photosynthesis in NatureAnnual Review of Plant Physiology and Plant Molecular Biology, 1994
- Recent advances in the understanding of chlorophyll excited state dynamics in thylakoid membranes and isolated reaction centre complexesJournal of Photochemistry and Photobiology B: Biology, 1992
- Photoprotection and Other Responses of Plants to High Light StressAnnual Review of Plant Physiology and Plant Molecular Biology, 1992
- Control of the light‐harvesting function of chloroplast membranes by aggregation of the LHCII chlorophyll—protein complexFEBS Letters, 1991