Differential roles of hydrogen peroxide and superoxide in mediating IL‐1‐induced NF‐κB activation and iNOS expression in bovine articular chondrocytes
- 6 December 2002
- journal article
- research article
- Published by Wiley in Journal of Cellular Biochemistry
- Vol. 88 (4), 783-793
- https://doi.org/10.1002/jcb.10428
Abstract
Our previous studies showed that reactive oxygen species (ROS) are required for the pro-inflammatory cytokine interleukin-1β (IL-1) to induce the activity of the Nuclear transcription Factor-kappaB (NF-κB) and the expression of the inducible isoform of the nitric oxide synthase (iNOS) in bovine articular chondrocytes. This study aimed at elucidating the role of hydrogen peroxide (H2O2) and the superoxide radical, two major ROS, in mediating those IL-1-induced responses. The results obtained show that chondrocytes produce both H2O2 and superoxide radical in response to IL-1. Treatment of the chondrocyte cultures with H2O2 alone did not induce NF-κB activation or iNOS expression. Addition of H2O2 simultaneously with IL-1 did neither enhance nor inhibit NF-κB activation and iNOS expression, relatively to treatment with IL-1 alone. Accordingly, treatment with catalase did not inhibit those IL-1-induced responses. Treatment with superoxide dismutase, however, effectively prevented IL-1-induced IκB-α degradation and iNOS expression. Taken together, the results obtained indicate that superoxide mediates IL-1-induced IκB-α degradation and the consequent NF-κB activation and iNOS expression in chondrocytes, whereas H2O2 does not seem to participate in those IL-1-induced responses. In conclusion, the present study identifies the superoxide radical as the ROS involved in mediating the IL-1-induced signaling pathway that leads to NF-κB activation and to the expression of NF-κB-dependent genes in bovine articular chondrocytes.Keywords
This publication has 44 references indexed in Scilit:
- Evidence Linking Chondrocyte Lipid Peroxidation to Cartilage Matrix Protein DegradationJournal of Biological Chemistry, 2000
- Superoxide enhances interleukin 1β–mediated transcription of the hepatocyte-inducible nitric oxide synthase geneGastroenterology, 2000
- Activation of NF-κB in Normal Rat Kidney Epithelial (NRK52E) Cells Is Mediated via a Redox-Insensitive, Calcium-Dependent PathwayToxicology and Applied Pharmacology, 1999
- H2O2 and Tumor Necrosis Factor-α Induce Differential Binding of the Redox-responsive Transcription Factors AP-1 and NF-κB to the Interleukin-8 Promoter in Endothelial and Epithelial CellsPublished by Elsevier BV ,1998
- Potentiation of nitric oxide synthase expression by superoxide in interleukin 1β‐stimulated rat mesangial cellsFEBS Letters, 1998
- Transient Overexpression of Catalase Does Not Inhibit TNF- or PMA-Induced NF-κB ActivationBiochemical and Biophysical Research Communications, 1995
- IL-1-Induced Nitric Oxide Inhibits Chondrocyte Proliferation via PGE2Experimental Cell Research, 1995
- Structure, Regulation and Function of NF-kappaBAnnual Review of Cell Biology, 1994
- Release of oxygen radicals by articular chondrocytes: A study of luminol-dependent chemiluminescence and hydrogen peroxide secretionJournal of Bone and Mineral Research, 1992
- Effects of recombinant human IL-I? on production of prostaglandin E2, leukotriene B4, NAG, and superoxide by human synovial cells and chondrocytesInflammation, 1991