Applications of Biochromatography in the Screening of Bioactive Natural Products
Open Access
- 1 February 2013
- journal article
- review article
- Published by Oxford University Press (OUP) in Journal of Chromatographic Science
- Vol. 51 (8), 780-790
- https://doi.org/10.1093/chromsci/bmt002
Abstract
Searching for bioactive compounds from natural resources such as plant materials has become a focus for study. Several models, such as animal (biofluid, organ and tissue) and cellular (several kinds of cell lines), have traditionally been used for this purpose. As a fast, economic and effective way to identify or predict bioactive compounds in complex matrices, biochromatography has developed rapidly during the past years. Combing the properties of traditional chromatography and biomaterials, biochromatographic analysis possesses features of simultaneous screening, separation and structural identification for active compounds in a complex matrix. According to the process, biochromatography can be divided into offline and online approaches. For offline bioextraction, the biomaterials are used as the extraction phase and followed by routine chromatographic analysis. For online biochromatography, the biomaterials are directly used as the stationary phase for chromatographic analysis. This paper reviews the applications of offline bioextraction followed by chromatographic analysis and online biochromatography, including molecular, cell membrane and cell, and artificial biomembrane chromatography in the screening or predicting active compounds from natural sources.This publication has 42 references indexed in Scilit:
- A Combined Peritoneal Macrophage/Cell Membrane Chromatography and Offline GC–MS Method for Screening Anti-Inflammatory Components from Chinese Traditional Medicine Houttuynia cordata Thunb.Chromatographia, 2011
- Association mechanism of four acetylcholinesterase inhibitors (AChEIs) with human serum albumin: A biochromatographic approachJournal of Pharmaceutical and Biomedical Analysis, 2008
- Macrophage biospecific extraction and high performance liquid chromatography for hypothesis of immunological active components in Cordyceps sinensisJournal of Pharmaceutical and Biomedical Analysis, 2007
- Screening and analysis of bioactive compounds in traditional Chinese medicines using cell extract and gas chromatography–mass spectrometryJournal of Pharmaceutical and Biomedical Analysis, 2007
- Live cell extraction and HPLC–MS analysis for predicting bioactive components of traditional Chinese medicinesJournal of Pharmaceutical and Biomedical Analysis, 2006
- Screening, analysis and in vitro vasodilatation of effective components from Ligusticum ChuanxiongLife Sciences, 2005
- Hypothesis of potential active components in Angelica sinensis by using biomembrane extraction and high performance liquid chromatographyJournal of Pharmaceutical and Biomedical Analysis, 2005
- Natural Products as Sources of New Drugs over the Period 1981−2002Journal of Natural Products, 2003
- Analysis of Bioactive Components in Traditional Chinese Medicines by Molecular Biochromatography with α1-Acid Glycoprotein Stationary PhaseJournal of Basic and Clinical Physiology and Pharmacology, 2000
- Relationship between immobilised artificial membrane chromatographic retention and the brain penetration of structurally diverse drugsJournal of Pharmaceutical and Biomedical Analysis, 1997