A case report of Anisakis pegreffii (Nematoda, Anisakidae) identified from archival paraffin sections of a Croatian patient
Open Access
- 1 December 2015
- journal article
- case report
- Published by Springer Science and Business Media LLC in BMC Infectious Diseases
- Vol. 16 (1), 1-5
- https://doi.org/10.1186/s12879-016-1401-x
Abstract
This is the first report of anisakiasis in a Croatian patient, evidenced from an archival paraffin-embedded and hematoxylin-eosin stained tissue section. Anisakiasis has been only suspected in the country based on previously detected anti-Anisakis IgE seroprevalence in the healthy coastal population, as well as an acute case where pathohistological and serological findings suggested the diseases, but the migrating larva has not been retrieved. Seventy years-old female, operated in 1998 for pulmonary carcinoma, was admitted to the General hospital Šibenik, Croatia in 2003, because of gastric pain and nausea that lasted for couple of days. She was showing good general condition, full mobility and lucidity, subfebrile status. Abdominal palpation inferred acute pain in paraumbilical and ileocecal region. Exploratory right pararectal laparotomy revealed a hardened, 5 cm-long structure, located intraluminally in the sigmoid colon, not perforating colon serosa. The process has been dissected and sent for patohistological diagnosis. Results showed a 2 mm-long whitish nematode spiralised in muscular layer of colon mucosa surrounded by granulomatous inflammation. After genomic DNA isolation of the nematode from the histological section, and amplification at the mitochondrial cytochrome oxidase 2 locus, etiological agent has been identified as Anisakis pegreffii. Used methodology suggests that screening of archival suspicious sections is feasible in order to study epidemiology of this zoonotic disease poorly recognised in Croatia.Keywords
Funding Information
- Hrvatska Zaklada za Znanost (5576)
This publication has 63 references indexed in Scilit:
- Anisakiasis and Gastroallergic Reactions Associated withAnisakis pegreffiiInfection, ItalyEmerging Infectious Diseases, 2013
- Spontaneous splenic rupture and Anisakis appendicitis presenting as abdominal pain: a case reportJournal of Medical Case Reports, 2012
- A Case of Duodenal Anisakiasis with Duodenal UlcerChonnam Medical Journal, 2012
- Synergism between Prior Anisakis simplex Infections and Intake of NSAIDs, on the Risk of Upper Digestive Bleeding: A Case-Control StudyPLoS Neglected Tropical Diseases, 2011
- First molecular identification of the zoonotic parasite Anisakis pegreffii(Nematoda: Anisakidae) in a paraffin-embedded granuloma taken from a case of human intestinal anisakiasis in ItalyBMC Infectious Diseases, 2011
- Anisakidae nematodes and Trypanorhyncha cestodes of hygienic importance infecting the king mackerel Scomberomorus cavalla (Osteichthyes: Scombridae) in BrazilVeterinary Parasitology, 2011
- Occurrence and infection dynamics of anisakid larvae in Scomber scombrus, Trachurus trachurus, Sardina pilchardus, and Engraulis encrasicolus from Tarragona (NE Spain)Food Control, 2010
- Diagnosing Human Anisakiasis: Recombinant Ani s 1 and Ani s 7 Allergens versus the UniCAP 100 Fluorescence Enzyme ImmunoassayClinical and Vaccine Immunology, 2010
- The Association of Anisakiasis in the Ascending Colon with Sigmoid Colon Cancer: CT Colonography FindingsKorean Journal of Radiology, 2008
- Anisakis simplex only provokes allergic symptoms when the worm parasitises the gastrointestinal tractZeitschrift für Parasitenkunde, 2004