A Genome-Wide Screen for Microdeletions Reveals Disruption of Polarity Complex Genes in Diverse Human Cancers
- 14 March 2010
- journal article
- Published by American Association for Cancer Research (AACR) in Cancer Research
- Vol. 70 (6), 2158-2164
- https://doi.org/10.1158/0008-5472.can-09-3458
Abstract
In a genome-wide screen of 684 cancer cell lines, we identified homozygous intragenic microdeletions involving genes encoding components of the apical-basal cell polarity complexes. Among these, PARD3 is disrupted in cell lines and primary tumors from squamous carcinomas and glioblastomas. Reconstituting PARD3 expression in both cell types restores tight junctions and retards contact-dependent proliferation. Searching specifically for small intragenic microdeletions using high-resolution genomic arrays may be complementary to other genomic deletion screens and resequencing efforts in identifying new tumor suppressor genes. Cancer Res; 70(6); 2158–64Other Versions
This publication has 21 references indexed in Scilit:
- Genomic Profiling of Atypical Meningiomas Associates Gain of 1q With Poor Clinical OutcomeJournal of Neuropathology and Experimental Neurology, 2009
- Polarity and Differential Inheritance—Universal Attributes of Life?Cell, 2008
- Deregulation of Scribble Promotes Mammary Tumorigenesis and Reveals a Role for Cell Polarity in CarcinomaCell, 2008
- An Integrated Genomic Analysis of Human Glioblastoma MultiformeScience, 2008
- Comprehensive genomic characterization defines human glioblastoma genes and core pathwaysNature, 2008
- Patterns of somatic mutation in human cancer genomesNature, 2007
- The Consensus Coding Sequences of Human Breast and Colorectal CancersScience, 2006
- Glioma Test Array for Use with Formalin-Fixed, Paraffin-Embedded Tissue: Array Comparative Genomic Hybridization Correlates with Loss of Heterozygosity and Fluorescence in Situ HybridizationThe Journal of Molecular Diagnostics, 2006
- Analysis of array CGH data: from signal ratio to gain and loss of DNA regionsBioinformatics, 2004
- Gene Expression Omnibus: NCBI gene expression and hybridization array data repositoryNucleic Acids Research, 2002