Microarray probe expression measures, data normalization and statistical validation
Open Access
- 21 August 2003
- journal article
- Published by Wiley in Comparative and Functional Genomics
- Vol. 4 (4), 442-446
- https://doi.org/10.1002/cfg.312
Abstract
DNA microarray technology is a high-throughput method for gaining information on gene function. Microarray technology is based on deposition/synthesis, in an ordered manner, on a solid surface, of thousands of EST sequences/genes/oligonucleotides. Due to the high number of generated datapoints, computational tools are essential in microarray data analysis and mining to grasp knowledge from experimental results. In this review, we will focus on some of the methodologies actually available to define gene expression intensity measures, microarray data normalization, and statistical validation of differential expression.Keywords
Funding Information
- Ministry of Education, Universities and Research of Italy (FIRB RBAU01JTHS, PRIN2001 2001057147)
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