Effect of Hop (Humulus lupulus L.) Flavonoids on Aromatase (Estrogen Synthase) Activity
- 22 March 2006
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 54 (8), 2938-2943
- https://doi.org/10.1021/jf053162t
Abstract
The aim of this work was to study the effect of the prenylflavonoids xanthohumol, isoxanthohumol, and 8-prenylnaringenin on the activity and expression of the enzyme aromatase (estrogen synthase). The effect of different kinds of beer containing these prenylflavonoids was also tested. Aromatase activity was determined by measuring the release of tritiated water during the conversion of [3H]androstenedione to estrone. Aromatase expression was determined by RT-PCR. This assay was carried out in choriocarcinoma-derived JAR cells. The tested prenylflavonoids were able to inhibit estrogen formation, and their IC50 values were determined, although no effect on aromatase expression was found. Lager beer, alcohol-free beer, stout beer, and xanthohumol-rich stout beer (200 μL/mL) significantly decreased aromatase activity. In conclusion, prenylflavonoids are able to modulate aromatase activity, decreasing estrogen synthesis, with relevance for the prevention and treatment of estrogen-dependent disorders such as breast cancer. Keywords: Aromatase; beer; estrogens; hop; isoxanthohumol; 8-prenylnaringenin, xanthohumolKeywords
This publication has 18 references indexed in Scilit:
- Beer constituents as potential cancer chemopreventive agentsEuropean Journal of Cancer, 2005
- Metabolism of xanthohumol and isoxanthohumol, prenylated flavonoids from hops (Humulus lupulus L.), by human liver microsomesJournal of Mass Spectrometry, 2005
- Bone marrow stem cells have the ability to populate the entire central nervous system into fully differentiated parenchymal microgliaThe FASEB Journal, 2004
- Wine and resveratrol: mechanisms of cancer prevention?European Journal of Cancer Prevention, 2003
- Structure–function studies of aromatase and its inhibitors: a progress reportThe Journal of Steroid Biochemistry and Molecular Biology, 2003
- Aromatase Inhibitors in Breast CancerNew England Journal of Medicine, 2003
- Aromatase—A Brief OverviewAnnual Review of Physiology, 2002
- Aromatase and its inhibitorsThe Journal of Steroid Biochemistry and Molecular Biology, 1999
- Placental estrogen synthetase (aromatase): Evidence for phosphatase-dependent inactivationBiochemical and Biophysical Research Communications, 1989
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976