Inhibition of Bacterial Ammonia Oxidation by Organohydrazines in Soil Microcosms
Open Access
- 1 January 2012
- journal article
- Published by Frontiers Media SA in Frontiers in Microbiology
- Vol. 3, 10
- https://doi.org/10.3389/fmicb.2012.00010
Abstract
Hydroxylamine oxidation by hydroxylamine oxidoreductase (HAO) is a key step for energy-yielding in support of the growth of ammonia-oxidizing bacteria (AOB). Organohydrazines have been shown to inactivate HAO from Nitrosomonas europaea, and may serve as selective inhibitors to differentiate bacterial from archaeal ammonia oxidation due to the absence of bacterial HAO gene homolog in known ammonia-oxidizing archaea (AOA). In this study, the effects of three organohydrazines on activity, abundance, and composition of AOB and AOA were evaluated in soil microcosms. The results indicate that phenylhydrazine and methylhydrazine at the concentration of 100 μmol g−1 dry weight soil completely suppressed the activity of soil nitrification. Denaturing gradient gel electrophoresis fingerprinting and sequencing analysis of bacterial ammonia monooxygenase subunit A gene (amoA) clearly demonstrated that nitrification activity change is well paralleled with the growth of Nitrosomonas europaea-like AOB in soil microcosms. No significant correlation between AOA community structure and nitrification activity was observed among all treatments during the incubation period, although incomplete inhibition of nitrification activity occurred in 2-hydroxyethylhydrazine-amended soil microcosms. These findings show that the HAO-targeted organohydrazines can effectively inhibit bacterial nitrification in soil, and the mechanism of organohydrazine affecting AOA remains unclear.This publication has 31 references indexed in Scilit:
- Nitrososphaera viennensis , an ammonia oxidizing archaeon from soilProceedings of the National Academy of Sciences of the United States of America, 2011
- Autotrophic ammonia oxidation by soil thaumarchaeaProceedings of the National Academy of Sciences of the United States of America, 2010
- Nitrosopumilus maritimus genome reveals unique mechanisms for nitrification and autotrophy in globally distributed marine crenarchaeaProceedings of the National Academy of Sciences of the United States of America, 2010
- Responses of Ammonia-Oxidizing Bacterial and Archaeal Populations to Organic Nitrogen Amendments in Low-Nutrient GroundwaterApplied and Environmental Microbiology, 2010
- Abundances of crenarchaeal amoA genes and transcripts in the Pacific OceanEnvironmental Microbiology, 2010
- Dynamics and functional relevance of ammonia‐oxidizing archaea in two agricultural soilsEnvironmental Microbiology, 2009
- Cultivation of a thermophilic ammonia oxidizing archaeon synthesizing crenarchaeolEnvironmental Microbiology, 2008
- An Earth-system perspective of the global nitrogen cycleNature, 2008
- Application of Real-Time PCR To Study Effects of Ammonium on Population Size of Ammonia-Oxidizing Bacteria in SoilApplied and Environmental Microbiology, 2004
- The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis toolsNucleic Acids Research, 1997