Persistence of virus and viral genome in myocardium after Coxsackievirus B3-induced murine myocarditis
- 1 April 1994
- journal article
- Published by Oxford University Press (OUP) in Clinical and Experimental Immunology
- Vol. 96 (1), 69-74
- https://doi.org/10.1111/j.1365-2249.1994.tb06232.x
Abstract
SUMMARY: Following infection with Coxsackievirus B3 (CVB3). A-strain mice develop ongoing myocarditis that persists after the virus ceases to be cultivatable from heart tissue. We studied the natural history of this virus-induced but apparently autoimmune inflammation by means of in situ hybridization (ISH) and by polymerase chain reaction (PCR). Both ISH and culture allowed detection of virus up to 2 weeks post-infection in virtually all heart tissues. In contrast, PCR revealed the presence of viral genome for a substantially longer period of time, i.e. at least 34 days after CVB3 infection. Similarly, the majority of mice showed myocardial inflammation at this time point. However, the persistence of virus did not correlate with ongoing myocarditis, and vice versa. Most mice with ongoing myocarditis produced heart myosin autoantibodies, most probably as a result of tissue damage. The lack of correlation between presence of ongoing inflammation and persistence of virus supports our previous view that the late phase of CVB3-induced myocarditis is mediated by auto immunological mechanisms.Keywords
This publication has 30 references indexed in Scilit:
- Experimentally-Induced Autoimmune Myocarditis: Production of Heart Myosin-Specific Autoantibodies Within the Inflammatory InfiltrateAutoimmunity, 1991
- The viral genome in experimental murine coxsackievirus B3 myocarditis: A Northern blotting anaylsisJournal of Molecular and Cellular Cardiology, 1990
- Molecular approaches to enteroviral diagnosis in idiopathic cardiomyopathy and myocarditisJournal of the American College of Cardiology, 1990
- Persistence of enteroviral RNA in chronic fatigue syndrome is associated with the abnormal production of equal amounts of positive and negative strands of enteroviral RNAJournal of General Virology, 1990
- Autoimmune myocarditis: a paradigm of post-infection autoimmune diseaseImmunology Today, 1988
- The natural history of encephalomyocarditis virus-induced myositis and myocarditis in mice. Viral persistence demonstrated by in situ hybridization.The Journal of Experimental Medicine, 1988
- Heart-specific autoantibodies induced by Coxsackievirus B3: Identification of heart autoantigensClinical Immunology and Immunopathology, 1987
- Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform ExtractionAnalytical Biochemistry, 1987
- In situ hybridization methods for the detection of somatostatin mRNA in tissue sections using antisense RNA probesJournal of Molecular Histology, 1986
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975