Epimorphic regeneration approach to tissue replacement in adult mammals
- 4 December 2009
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 107 (8), 3351-3355
- https://doi.org/10.1073/pnas.0905851106
Abstract
Urodeles and fetal mammals are capable of impressive epimorphic regeneration in a variety of tissues, whereas the typical default response to injury in adult mammals consists of inflammation and scar tissue formation. One component of epimorphic regeneration is the recruitment of resident progenitor and stem cells to a site of injury. Bioactive molecules resulting from degradation of extracellular matrix (ECM) have been shown to recruit a variety of progenitor and stem cells in vitro in adult mammals. The ability to recruit multipotential cells to the site of injury by in vivo administration of chemotactic ECM degradation products in a mammalian model of digit amputation was investigated in the present study. Adult, 6- to 8-week-old C57/BL6 mice were subjected to midsecond phalanx amputation of the third digit of the right hind foot and either treated with chemotactic ECM degradation products or left untreated. At 14 days after amputation, mice treated with ECM degradation products showed an accumulation of heterogeneous cells that expressed markers of multipotency, including Sox2, Sca1, and Rex1 (Zfp42). Cells isolated from the site of amputation were capable of differentiation along neuroectodermal and mesodermal lineages, whereas cells isolated from control mice were capable of differentiation along only mesodermal lineages. The present findings demonstrate the recruitment of endogenous stem cells to a site of injury, and/or their generation/proliferation therein, in response to ECM degradation products.Keywords
This publication has 59 references indexed in Scilit:
- Macrophage Participation in the Degradation and Remodeling of Extracellular Matrix ScaffoldsTissue Engineering, Part A, 2009
- Chemoattraction of Progenitor Cells by Remodeling Extracellular Matrix ScaffoldsTissue Engineering, Part A, 2009
- Macrophage phenotype and remodeling outcomes in response to biologic scaffolds with and without a cellular componentBiomaterials, 2009
- Chemoattractant activity of degradation products of fetal and adult skin extracellular matrix for keratinocyte progenitor cellsJournal of Tissue Engineering and Regenerative Medicine, 2008
- A Perivascular Origin for Mesenchymal Stem Cells in Multiple Human OrgansCell Stem Cell, 2008
- SOX2-expressing progenitor cells generate all of the major cell types in the adult mouse pituitary glandProceedings of the National Academy of Sciences, 2008
- Fragments of extracellular matrix as mediators of inflammationThe International Journal of Biochemistry & Cell Biology, 2007
- Liver regenerationJournal of Cellular Physiology, 2007
- Antibacterial Activity within Degradation Products of Biological Scaffolds Composed of Extracellular MatrixTissue Engineering, 2006
- Neural Crest As the Source of Adult Stem CellsStem Cells and Development, 2006