Chitosan Acetate Bandage as a Topical Antimicrobial Dressing for Infected Burns
- 1 February 2009
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 53 (2), 393-400
- https://doi.org/10.1128/aac.00760-08
Abstract
An engineered chitosan acetate bandage preparation (HemCon) is used as a hemostatic dressing, and its chemical structure suggests that it should also be antimicrobial. We previously showed that when a chitosan acetate bandage was applied to full-thickness excisional wounds in mice that had been infected with pathogenic bioluminescent bacteria (Pseudomonas aeruginosa, Proteus mirabilis, andStaphylococcus aureus), it was able to rapidly kill the bacteria and save the mice from developing fatal infections. Wound healing was also stimulated. In the present study, we asked whether a chitosan acetate bandage could act as a topical antimicrobial dressing when it was applied to third-degree burns in mice contaminated with two of these bacterial species (P. aeruginosaandP. mirabilis). Preliminary experiments established the length of burn time and the number of bacteria needed to produce fatal infections in untreated mice and established that the chitosan acetate bandage could adhere to the infected burn for up to 21 days. In the case ofP. aeruginosainfections, the survival rate of mice treated with the chitosan acetate bandage was 73.3% (whereas the survival rate of mice treated with a nanocrystalline silver dressing was 27.3% [P= 0.0055] and that of untreated mice was 13.3% [P< 0.0002]). ForP. mirabilisinfections, the comparable survival rates were 66.7%, 62.5%, and 23.1% respectively. Quantitative bioluminescent signals showed that the chitosan acetate bandage effectively controlled the growth of bacteria in the burn and prevented the development of systemic sepsis, as shown by blood culture. These data suggest that chitosan acetate bandage is efficacious in preventing fatal burn infections.This publication has 40 references indexed in Scilit:
- Effect of chitosan acetate bandage on wound healing in infected and noninfected wounds in miceWound Repair and Regeneration, 2008
- Infections in BurnsThe Journal of Trauma and Acute Care Surgery, 2007
- Use of chitosan bandage to prevent fatal infections developing from highly contaminated wounds in miceBiomaterials, 2006
- Burn Wound InfectionsClinical Microbiology Reviews, 2006
- Monitoring photodynamic therapy of localized infections by bioluminescence imaging of genetically engineered bacteriaJournal of Photochemistry and Photobiology B: Biology, 2005
- Chitosan delivery systems for the treatment of oral mucositis: in vitro and in vivo studiesJournal of Controlled Release, 2004
- Rapid Control of Wound Infections by Targeted Photodynamic Therapy Monitored by In Vivo Bioluminescence Imaging¶Photochemistry and Photobiology, 2002
- Calculation of a new Meeh constant and experimental determination of burn sizeBurns, 1996
- Relationship Between Extent of Burn Injury and Magnitude of Microbial Translocation From the IntestineJournal of Burn Care & Rehabilitation, 1993
- Surface Infection with Pseudomonas AeruginosaAnnals of Surgery, 1964