Notch-dependent downregulation of the homeodomain gene cut is required for the mitotic cycle/endocycle switch and cell differentiation inDrosophilafollicle cells
- 1 October 2005
- journal article
- Published by The Company of Biologists in Development
- Vol. 132 (19), 4299-4308
- https://doi.org/10.1242/dev.02015
Abstract
During Drosophila mid-oogenesis, follicular epithelial cells switch from the mitotic cycle to the specialized endocycle in which the M phase is skipped. The switch, along with cell differentiation in follicle cells, is induced by Notch signaling. We show that the homeodomain gene cut functions as a linker between Notch and genes that are involved in cell-cycle progression. Cut was expressed in proliferating follicle cells but not in cells in the endocycle. Downregulation of Cut expression was controlled by the Notch pathway and was essential for follicle cells to differentiate and to enter the endocycle properly. cut-mutant follicle cells entered the endocycle and differentiated prematurely in a cell-autonomous manner. By contrast, prolonged expression of Cut caused defects in the mitotic cycle/endocycle switch. These cells continued to express an essential mitotic cyclin, Cyclin A, which is normally degraded by the Fizzy-related-APC/C ubiquitin proteosome system during the endocycle. Cut promoted Cyclin A expression by negatively regulating Fizzy-related. Our data suggest that Cut functions in regulating both cell differentiation and the cell cycle, and that downregulation of Cut by Notch contributes to the mitotic cycle/endocycle switch and cell differentiation in follicle cells.Keywords
This publication has 59 references indexed in Scilit:
- Fringe‐dependent notch activation and tramtrack function are required for specification of the polar cells in Drosophila oogenesisDevelopmental Dynamics, 2005
- The mitotic-to-endocycle switch inDrosophilafollicle cells is executed by Notch-dependent regulation of G1/S, G2/M and M/G1 cell-cycle transitionsDevelopment, 2004
- DrosophiladMyc is required for ovary cell growth and endoreplicationDevelopment, 2004
- Tumor suppressor pRB functions as a co‐repressor of the CCAAT displacement protein (CDP/cut) to regulate cell cycle controlled histone H4 transcriptionJournal of Cellular Physiology, 2003
- CDP/Cux Stimulates Transcription from the DNA Polymerase α Gene PromoterMolecular and Cellular Biology, 2003
- NOTCH ANDPRESENILIN: Regulated Intramembrane Proteolysis Links Development and DegenerationAnnual Review of Neuroscience, 2003
- Transcriptional Repressor Functions of Drosophila E2F1 and E2F2 Cooperate To Inhibit Genomic DNA Synthesis in Ovarian Follicle CellsMolecular and Cellular Biology, 2003
- Notch Signaling: Cell Fate Control and Signal Integration in DevelopmentScience, 1999
- Reversible histone modification and the chromosome cell cycleBioEssays, 1992
- The choice of cell fate in the epidermis of DrosophilaCell, 1991