Contractile response to low peroxide concentrations: myofibrillar calcium sensitivity as a likely target for redox‐modulation of skeletal muscle function
- 8 December 2000
- journal article
- Published by Wiley in The FASEB Journal
- Vol. 15 (2), 309-311
- https://doi.org/10.1096/fj.00-0507fje
Abstract
Endogenous peroxides and related reactive oxygen species may influence various steps in the contractile process. Single mouse skeletal muscle fibers were used to study the effects of hydrogen peroxide (H2O2) and t-butyl hydroperoxide (t-BOOH) on force and myoplasmic Ca2+ concentration ([Ca2+]i). Both peroxides (1010 to 105 M) decreased tetanic [Ca2+]i and increased force during submaximal tetani. Catalase (1 kU/ml) blocked the effect of H2O2, but not of t-BOOH. The decrease in tetanic [Ca2+]i was constant, while the effect on force was biphasic: A transitory increase was followed by a steady decline to the initial level. Myofibrillar Ca2+ sensitivity remained increased during incubation with either peroxide. Only the highest peroxide concentration (10 mM) increased resting [Ca2+]i and slowed the return of [Ca2+]i to its resting level after a contraction, evidence of impaired sarcoplasmic reticulum Ca2+ re-uptake. The peroxides increased maximal force production and the rate of force redevelopment, and decreased maximum shortening velocity. N-ethylmaleimide (25 mM, thiol-alkylating agent) prevented the response to 1 mM H2O2. These results show that myofibrillar Ca2+ sensitivity and cross-bridge kinetics are influenced by H2O2 and t-BOOH concentrations that approach those found physiologically, and these findings indicate a role for endogenous oxidants in the regulation of skeletal muscle function.Keywords
This publication has 34 references indexed in Scilit:
- Effect of hydrogen peroxide and dithiothreitol on contractile function of single skeletal muscle fibres from the mouseThe Journal of Physiology, 1998
- Multiple Classes of Sulfhydryls Modulate the Skeletal Muscle Ca2+ Release ChannelPublished by Elsevier BV ,1997
- Oxidants as Stimulators of Signal TransductionFree Radical Biology & Medicine, 1997
- Mechanisms underlying changes of tetanic [Ca2+]i and force in skeletal muscleActa Physiologica Scandinavica, 1996
- Hydrogen Peroxide Stimulates the Ca2+ Release Channel from Skeletal Muscle Sarcoplasmic ReticulumPublished by Elsevier BV ,1995
- Calcium homeostasis in rabbit ventricular myocytes. Disruption by hypochlorous acid and restoration by dithiothreitol.Circulation Research, 1991
- Decreased carbon centered and hydrogen radicals in skeletal muscle of vitamin E supplemented ratsBiochemical and Biophysical Research Communications, 1991
- Cell calcium, vitamin E, and teh thiol redox system in cytocoxicityFree Radical Biology & Medicine, 1989
- Electron spin resonance studies of intact mammalian skeletal muscleBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1985
- Free radicals and tissue damage produced by exerciseBiochemical and Biophysical Research Communications, 1982